Deformable Models for Segmentation of CLSM Tissue Images and Its Application in FISH Signal Analysis
In this paper we present an application of deformable models for the segmentation of volumetric tissue images. The three‐dimensional images are obtained using confocal microscope. The segmented images have been used for the quantitative analysis of the Fluorescence In Situ Hybridization (FISH) signa...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1999-01-01
|
| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1999/350317 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548012163858432 |
|---|---|
| author | P. S. Umesh Adiga B. B. Chaudhuri |
| author_facet | P. S. Umesh Adiga B. B. Chaudhuri |
| author_sort | P. S. Umesh Adiga |
| collection | DOAJ |
| description | In this paper we present an application of deformable models for the segmentation of volumetric tissue images. The three‐dimensional images are obtained using confocal microscope. The segmented images have been used for the quantitative analysis of the Fluorescence In Situ Hybridization (FISH) signals. An ellipsoidal surface initialized around the cell of interest acts as a deformable model. The deformable model surface voxels are subjected to various internal and external forces derived from underlying image features as well as externally imposed constraints. The deformable model converges to the optimum cell shape when the vector sum of all the forces acting on the model is zero. The result of segmentation is used to confirm the cell membership of the FISH signals and to reject all the signals that lie outside the cell nuclei. Three‐dimensional region isolation and labeling technique is used to label and count the FISH signals per cell nucleus. A simple study on the effect of different segmentation methods over a quantitative analysis of FISH signals is also presented. |
| format | Article |
| id | doaj-art-c44bb2e1ac83421e8f6d5710e936318f |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1999-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-c44bb2e1ac83421e8f6d5710e936318f2025-02-03T06:42:28ZengWileyAnalytical Cellular Pathology0921-89121878-36511999-01-0118421122510.1155/1999/350317Deformable Models for Segmentation of CLSM Tissue Images and Its Application in FISH Signal AnalysisP. S. Umesh Adiga0B. B. Chaudhuri1Indian Statistical Institute, Calcutta 700035, IndiaIndian Statistical Institute, Calcutta 700035, IndiaIn this paper we present an application of deformable models for the segmentation of volumetric tissue images. The three‐dimensional images are obtained using confocal microscope. The segmented images have been used for the quantitative analysis of the Fluorescence In Situ Hybridization (FISH) signals. An ellipsoidal surface initialized around the cell of interest acts as a deformable model. The deformable model surface voxels are subjected to various internal and external forces derived from underlying image features as well as externally imposed constraints. The deformable model converges to the optimum cell shape when the vector sum of all the forces acting on the model is zero. The result of segmentation is used to confirm the cell membership of the FISH signals and to reject all the signals that lie outside the cell nuclei. Three‐dimensional region isolation and labeling technique is used to label and count the FISH signals per cell nucleus. A simple study on the effect of different segmentation methods over a quantitative analysis of FISH signals is also presented.http://dx.doi.org/10.1155/1999/350317 |
| spellingShingle | P. S. Umesh Adiga B. B. Chaudhuri Deformable Models for Segmentation of CLSM Tissue Images and Its Application in FISH Signal Analysis Analytical Cellular Pathology |
| title | Deformable Models for Segmentation of CLSM Tissue Images and Its Application in FISH Signal Analysis |
| title_full | Deformable Models for Segmentation of CLSM Tissue Images and Its Application in FISH Signal Analysis |
| title_fullStr | Deformable Models for Segmentation of CLSM Tissue Images and Its Application in FISH Signal Analysis |
| title_full_unstemmed | Deformable Models for Segmentation of CLSM Tissue Images and Its Application in FISH Signal Analysis |
| title_short | Deformable Models for Segmentation of CLSM Tissue Images and Its Application in FISH Signal Analysis |
| title_sort | deformable models for segmentation of clsm tissue images and its application in fish signal analysis |
| url | http://dx.doi.org/10.1155/1999/350317 |
| work_keys_str_mv | AT psumeshadiga deformablemodelsforsegmentationofclsmtissueimagesanditsapplicationinfishsignalanalysis AT bbchaudhuri deformablemodelsforsegmentationofclsmtissueimagesanditsapplicationinfishsignalanalysis |