Induction of an anti-tumor immune response by sonodynamic therapy, combining 5-aminolevulinic acid and high-intensity focused ultrasound using the trigger pulse sonication
Ultrasound-based cancer therapies offer non-invasive treatment options for deep-seated tumors, yet their clinical application is often limited by safety concerns. High-intensity focused ultrasound (HIFU) has demonstrated therapeutic potential, but its high-power requirements can damage surrounding h...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-12-01
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| Series: | WFUMB Ultrasound Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949668325000102 |
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| author | Tohru Satoh Rui Tada Taiki Yamaguchi Yoko Endo-Takahashi Takashi Kanno Mai Hayakawa Yoshiyuki Adachi Yoichi Negishi Jun Okamoto Shin Yoshizawa Ken Masamune |
| author_facet | Tohru Satoh Rui Tada Taiki Yamaguchi Yoko Endo-Takahashi Takashi Kanno Mai Hayakawa Yoshiyuki Adachi Yoichi Negishi Jun Okamoto Shin Yoshizawa Ken Masamune |
| author_sort | Tohru Satoh |
| collection | DOAJ |
| description | Ultrasound-based cancer therapies offer non-invasive treatment options for deep-seated tumors, yet their clinical application is often limited by safety concerns. High-intensity focused ultrasound (HIFU) has demonstrated therapeutic potential, but its high-power requirements can damage surrounding healthy tissues, particularly near sensitive structures such as the gastrointestinal tract. The optimal balance between therapeutic efficacy and safety remains a significant challenge in ultrasound-based cancer treatment. Here we show that combining 5-aminolevulinic acid (5-ALA)-mediated sonodynamic therapy (SDT) with a novel HIFU system achieves effective tumor suppression at reduced acoustic intensities while enhancing anti-tumor immune responses. This novel system features the use of a trigger HIFU sequence that enables precise control over cavitation bubbles within the target tissue. In a murine colon carcinoma model, this approach demonstrated significant tumor growth inhibition with single-session treatment comparable to multiple HIFU sessions, while increasing tumor infiltration by activated CD8+ T cells and antigen-presenting dendritic cells. These findings extend beyond previous studies by revealing that SDT not only enables effective tumor treatment at lower, safer acoustic intensities but also promotes beneficial immune responses that could enhance therapeutic outcomes. This combination of reduced treatment intensity, shortened duration, and immune system activation suggests potential applications for treating tumors in anatomically sensitive locations. The demonstrated immunological effects also indicate possible synergies with existing cancer immunotherapies, potentially expanding treatment options for patients with deep-seated solid tumors. |
| format | Article |
| id | doaj-art-c4174d8a792447f5b36c3e9cd25c37ac |
| institution | OA Journals |
| issn | 2949-6683 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | WFUMB Ultrasound Open |
| spelling | doaj-art-c4174d8a792447f5b36c3e9cd25c37ac2025-08-20T02:01:58ZengElsevierWFUMB Ultrasound Open2949-66832025-12-013210008810.1016/j.wfumbo.2025.100088Induction of an anti-tumor immune response by sonodynamic therapy, combining 5-aminolevulinic acid and high-intensity focused ultrasound using the trigger pulse sonicationTohru Satoh0Rui Tada1Taiki Yamaguchi2Yoko Endo-Takahashi3Takashi Kanno4Mai Hayakawa5Yoshiyuki Adachi6Yoichi Negishi7Jun Okamoto8Shin Yoshizawa9Ken Masamune10Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, Japan; Department of Drug Delivery and Molecular Biopharmaceutics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan; SONIRE Therapeutics Inc., Tokyo, Japan; Corresponding author. Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-Cho, Shinjuku-ku, Tokyo 162-8666, Japan.Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, JapanDepartment of Drug Delivery and Molecular Biopharmaceutics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, JapanDepartment of Drug Delivery and Molecular Biopharmaceutics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, JapanLaboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, JapanLaboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, JapanLaboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, JapanDepartment of Drug Delivery and Molecular Biopharmaceutics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, JapanSONIRE Therapeutics Inc., Tokyo, JapanCommunications Engineering, Tohoku University Graduate School of Engineering, Sendai, Japan; SONIRE Therapeutics Inc., Tokyo, JapanAdvanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, JapanUltrasound-based cancer therapies offer non-invasive treatment options for deep-seated tumors, yet their clinical application is often limited by safety concerns. High-intensity focused ultrasound (HIFU) has demonstrated therapeutic potential, but its high-power requirements can damage surrounding healthy tissues, particularly near sensitive structures such as the gastrointestinal tract. The optimal balance between therapeutic efficacy and safety remains a significant challenge in ultrasound-based cancer treatment. Here we show that combining 5-aminolevulinic acid (5-ALA)-mediated sonodynamic therapy (SDT) with a novel HIFU system achieves effective tumor suppression at reduced acoustic intensities while enhancing anti-tumor immune responses. This novel system features the use of a trigger HIFU sequence that enables precise control over cavitation bubbles within the target tissue. In a murine colon carcinoma model, this approach demonstrated significant tumor growth inhibition with single-session treatment comparable to multiple HIFU sessions, while increasing tumor infiltration by activated CD8+ T cells and antigen-presenting dendritic cells. These findings extend beyond previous studies by revealing that SDT not only enables effective tumor treatment at lower, safer acoustic intensities but also promotes beneficial immune responses that could enhance therapeutic outcomes. This combination of reduced treatment intensity, shortened duration, and immune system activation suggests potential applications for treating tumors in anatomically sensitive locations. The demonstrated immunological effects also indicate possible synergies with existing cancer immunotherapies, potentially expanding treatment options for patients with deep-seated solid tumors.http://www.sciencedirect.com/science/article/pii/S2949668325000102high-intensity focused ultrasoundsonodynamic therapy5-Aminolevulinic acidprotoporphyrin IXreactive oxygen speciestumor immunity |
| spellingShingle | Tohru Satoh Rui Tada Taiki Yamaguchi Yoko Endo-Takahashi Takashi Kanno Mai Hayakawa Yoshiyuki Adachi Yoichi Negishi Jun Okamoto Shin Yoshizawa Ken Masamune Induction of an anti-tumor immune response by sonodynamic therapy, combining 5-aminolevulinic acid and high-intensity focused ultrasound using the trigger pulse sonication WFUMB Ultrasound Open high-intensity focused ultrasound sonodynamic therapy 5-Aminolevulinic acid protoporphyrin IX reactive oxygen species tumor immunity |
| title | Induction of an anti-tumor immune response by sonodynamic therapy, combining 5-aminolevulinic acid and high-intensity focused ultrasound using the trigger pulse sonication |
| title_full | Induction of an anti-tumor immune response by sonodynamic therapy, combining 5-aminolevulinic acid and high-intensity focused ultrasound using the trigger pulse sonication |
| title_fullStr | Induction of an anti-tumor immune response by sonodynamic therapy, combining 5-aminolevulinic acid and high-intensity focused ultrasound using the trigger pulse sonication |
| title_full_unstemmed | Induction of an anti-tumor immune response by sonodynamic therapy, combining 5-aminolevulinic acid and high-intensity focused ultrasound using the trigger pulse sonication |
| title_short | Induction of an anti-tumor immune response by sonodynamic therapy, combining 5-aminolevulinic acid and high-intensity focused ultrasound using the trigger pulse sonication |
| title_sort | induction of an anti tumor immune response by sonodynamic therapy combining 5 aminolevulinic acid and high intensity focused ultrasound using the trigger pulse sonication |
| topic | high-intensity focused ultrasound sonodynamic therapy 5-Aminolevulinic acid protoporphyrin IX reactive oxygen species tumor immunity |
| url | http://www.sciencedirect.com/science/article/pii/S2949668325000102 |
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