‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study

‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study

Large DNA molecules (>20 kb) are difficult analytes prone to breakage during serial manipulations and cannot be ‘rescued’ as full-length amplicons. Accordingly, to present, modify and analyze arrays of large, single DNA molecules, we created an easily realizable approach offering gentle confineme...

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Bibliographic Details
Main Authors: Susana Calle-Casteñeda, Eamon Winden, Alejandro Vasquez-Echeverri, Matthew Schickling, Evelyn Browning, Juan Pablo Hernandez Ortiz, David C Schwartz
Format: Article
Language:English
Published: Taylor & Francis Group 2024-06-01
Series:BioTechniques
Subjects:
confinement
epifluorescence microscopy
Fick's second law
hydrogels
large DNA molecule arrays
reagent transport
Online Access:https://www.tandfonline.com/doi/10.2144/btn-2023-0123
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https://www.tandfonline.com/doi/10.2144/btn-2023-0123

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