Protocol for quantification of intercellular connection transmission in ebolavirus infections using ImageJ

Summary: Previous work demonstrates that ebolaviruses can spread to neighboring cells through intercellular connections. Here, we present a protocol to quantify the intercellular spread of ebolaviruses via immunofluorescence. We describe steps for cell plating, Bundibugyo virus infection, and adding...

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Bibliographic Details
Main Authors: Rodrigo I. Santos, Alexander Bukreyev
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005288
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