Synthesis and Cytotoxic Activity of Conjugates of Mitochondrial-Directed Cationic Compound F16 with Ursane-Structure Triterpenic Acids Containing a Polyhydroxylated A-ring

We chemically linked corosolic and asiatic acids and a synthetic polyoxygenated analogue of ursolic acid via an alkyl linker to the cationic mitochondrial targeting compound F16 (4-(1H-indole-3-ylvinyl)-<i>N</i>-methylpyridinium iodide). The conjugates were tested for cytotoxic activity...

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Main Authors: Anna Spivak, Darya Nedopekina, Eldar Davletshin
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:Chemistry Proceedings
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Online Access:https://www.mdpi.com/2673-4583/14/1/43
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author Anna Spivak
Darya Nedopekina
Eldar Davletshin
author_facet Anna Spivak
Darya Nedopekina
Eldar Davletshin
author_sort Anna Spivak
collection DOAJ
description We chemically linked corosolic and asiatic acids and a synthetic polyoxygenated analogue of ursolic acid via an alkyl linker to the cationic mitochondrial targeting compound F16 (4-(1H-indole-3-ylvinyl)-<i>N</i>-methylpyridinium iodide). The conjugates were tested for cytotoxic activity against two human lung adenocarcinoma cell lines, H1299 and A549, and non-cancerous mouse embryonic fibroblast cells. The results showed that conjugation of polyoxygenated triterpene acids with the terminal cationic fragment F16 in the C-28 side chain enhanced cytotoxicity (30–35 fold) compared to the original natural ursolic acid. However, the presence of hydroxyl or acetyl functions in the A-ring of F16 conjugates of corosolic or asiatic acids resulted in a significant decrease in cytotoxicity compared to their structural analogue, the F16 derivative of ursolic acid.
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spelling doaj-art-c23b210734b14ca79ca596123273ae472025-08-20T02:56:06ZengMDPI AGChemistry Proceedings2673-45832023-11-011414310.3390/ecsoc-27-16177Synthesis and Cytotoxic Activity of Conjugates of Mitochondrial-Directed Cationic Compound F16 with Ursane-Structure Triterpenic Acids Containing a Polyhydroxylated A-ringAnna Spivak0Darya Nedopekina1Eldar Davletshin2Institute of Petrochemistry and Catalysis, Russian Academy of Sciences, 141 Prospekt Oktyabrya, Ufa 450075, RussiaInstitute of Petrochemistry and Catalysis, Russian Academy of Sciences, 141 Prospekt Oktyabrya, Ufa 450075, RussiaInstitute of Petrochemistry and Catalysis, Russian Academy of Sciences, 141 Prospekt Oktyabrya, Ufa 450075, RussiaWe chemically linked corosolic and asiatic acids and a synthetic polyoxygenated analogue of ursolic acid via an alkyl linker to the cationic mitochondrial targeting compound F16 (4-(1H-indole-3-ylvinyl)-<i>N</i>-methylpyridinium iodide). The conjugates were tested for cytotoxic activity against two human lung adenocarcinoma cell lines, H1299 and A549, and non-cancerous mouse embryonic fibroblast cells. The results showed that conjugation of polyoxygenated triterpene acids with the terminal cationic fragment F16 in the C-28 side chain enhanced cytotoxicity (30–35 fold) compared to the original natural ursolic acid. However, the presence of hydroxyl or acetyl functions in the A-ring of F16 conjugates of corosolic or asiatic acids resulted in a significant decrease in cytotoxicity compared to their structural analogue, the F16 derivative of ursolic acid.https://www.mdpi.com/2673-4583/14/1/43ursolic acidconjugatespolyhydroxylated A-ringdelocalized lypophilic cationsF16anti-cancer agents
spellingShingle Anna Spivak
Darya Nedopekina
Eldar Davletshin
Synthesis and Cytotoxic Activity of Conjugates of Mitochondrial-Directed Cationic Compound F16 with Ursane-Structure Triterpenic Acids Containing a Polyhydroxylated A-ring
Chemistry Proceedings
ursolic acid
conjugates
polyhydroxylated A-ring
delocalized lypophilic cations
F16
anti-cancer agents
title Synthesis and Cytotoxic Activity of Conjugates of Mitochondrial-Directed Cationic Compound F16 with Ursane-Structure Triterpenic Acids Containing a Polyhydroxylated A-ring
title_full Synthesis and Cytotoxic Activity of Conjugates of Mitochondrial-Directed Cationic Compound F16 with Ursane-Structure Triterpenic Acids Containing a Polyhydroxylated A-ring
title_fullStr Synthesis and Cytotoxic Activity of Conjugates of Mitochondrial-Directed Cationic Compound F16 with Ursane-Structure Triterpenic Acids Containing a Polyhydroxylated A-ring
title_full_unstemmed Synthesis and Cytotoxic Activity of Conjugates of Mitochondrial-Directed Cationic Compound F16 with Ursane-Structure Triterpenic Acids Containing a Polyhydroxylated A-ring
title_short Synthesis and Cytotoxic Activity of Conjugates of Mitochondrial-Directed Cationic Compound F16 with Ursane-Structure Triterpenic Acids Containing a Polyhydroxylated A-ring
title_sort synthesis and cytotoxic activity of conjugates of mitochondrial directed cationic compound f16 with ursane structure triterpenic acids containing a polyhydroxylated a ring
topic ursolic acid
conjugates
polyhydroxylated A-ring
delocalized lypophilic cations
F16
anti-cancer agents
url https://www.mdpi.com/2673-4583/14/1/43
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AT daryanedopekina synthesisandcytotoxicactivityofconjugatesofmitochondrialdirectedcationiccompoundf16withursanestructuretriterpenicacidscontainingapolyhydroxylatedaring
AT eldardavletshin synthesisandcytotoxicactivityofconjugatesofmitochondrialdirectedcationiccompoundf16withursanestructuretriterpenicacidscontainingapolyhydroxylatedaring