Sleep habits, brain functional networks, and type 2 diabetes risk: cohort studies from North American and Asian populations and mediating Mendelian randomization analyses

Abstract Background Brain resting-state functional networks and sleep habits are associated with Type 2 diabetes mellitus (T2DM). However, the causal mediating effect between them remains largely unexplored. Methods We analyzed data from 20,994 U.S. adults in the National Health and Nutrition Examin...

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Main Authors: Faquan Hu, Qian Gong, Liyuan Xiong, Wei Zhao, Lingxiu Li, Yujiao Zheng
Format: Article
Language:English
Published: BMC 2025-06-01
Series:Diabetology & Metabolic Syndrome
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Online Access:https://doi.org/10.1186/s13098-025-01777-7
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author Faquan Hu
Qian Gong
Liyuan Xiong
Wei Zhao
Lingxiu Li
Yujiao Zheng
author_facet Faquan Hu
Qian Gong
Liyuan Xiong
Wei Zhao
Lingxiu Li
Yujiao Zheng
author_sort Faquan Hu
collection DOAJ
description Abstract Background Brain resting-state functional networks and sleep habits are associated with Type 2 diabetes mellitus (T2DM). However, the causal mediating effect between them remains largely unexplored. Methods We analyzed data from 20,994 U.S. adults in the National Health and Nutrition Examination Survey (NHANES) and 8,652 Chinese adults from the China Health and Aging Longitudinal Study (CHARLS), focusing on sleep habits and the incidence of T2DM. Two-sample Mendelian randomization (MR) was employed to assess the causal relationships between sleep habits, resting-state functional magnetic resonance imaging (rs-fMRI), and T2DM. Furthermore, a mediation analysis was conducted to examine the role of rs-fMRI in moderating the association between sleep patterns and T2DM. Results In the Chinese population, daytime naps lasting 0–0.25 h (β = 0.365) and more than 0.5 h (β = 0.039), along with nighttime sleep of less than 5 h (β = 0.049) and more than 9 h of sleep overall (β = 0.124) were linked to a higher risk of T2DM. Similarly, in the U.S. population, sleeping less than 5 h (β = 0.056), 5–6 h (β = 0.034), and more than 8 h (β = 0.052) also significantly increased the risk of T2DM. Moreover, sleeping in later than 1:00 a.m. or before 10:00 p.m. raises the chance of acquiring T2DM. A correlation between the incidence of T2DM and increased connectivity in the default mode network (DMN), visual network (VN), central executive network, (CEN), and motor networks (MN) was found by MR analysis. Additionally, mediation analysis revealed that sleep disorders, naps during the day, and daytime sleep affect T2DM morbidity via changing connectivity in the central executive, motor, and default mode networks. Conclusions According to our research, changed brain functional network connectivity and sleep habits, significantly influence the risk of T2DM. This demonstrates the critical function that brain networks play in modulating the link between sleep patterns and the prevalence of T2DM.
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spelling doaj-art-c1a6925b771c4b2da29b2a63bb7337692025-08-20T02:06:35ZengBMCDiabetology & Metabolic Syndrome1758-59962025-06-0117111510.1186/s13098-025-01777-7Sleep habits, brain functional networks, and type 2 diabetes risk: cohort studies from North American and Asian populations and mediating Mendelian randomization analysesFaquan Hu0Qian Gong1Liyuan Xiong2Wei Zhao3Lingxiu Li4Yujiao Zheng5College of Traditional Chinese Medicine, Anhui University of Chinese MedicineCollege of Traditional Chinese Medicine, Anhui University of Chinese MedicineCollege of Traditional Chinese Medicine, Anhui University of Chinese MedicineCollege of Traditional Chinese Medicine, Anhui University of Chinese MedicineCollege of Traditional Chinese Medicine, Anhui University of Chinese MedicineCollege of Traditional Chinese Medicine, Anhui University of Chinese MedicineAbstract Background Brain resting-state functional networks and sleep habits are associated with Type 2 diabetes mellitus (T2DM). However, the causal mediating effect between them remains largely unexplored. Methods We analyzed data from 20,994 U.S. adults in the National Health and Nutrition Examination Survey (NHANES) and 8,652 Chinese adults from the China Health and Aging Longitudinal Study (CHARLS), focusing on sleep habits and the incidence of T2DM. Two-sample Mendelian randomization (MR) was employed to assess the causal relationships between sleep habits, resting-state functional magnetic resonance imaging (rs-fMRI), and T2DM. Furthermore, a mediation analysis was conducted to examine the role of rs-fMRI in moderating the association between sleep patterns and T2DM. Results In the Chinese population, daytime naps lasting 0–0.25 h (β = 0.365) and more than 0.5 h (β = 0.039), along with nighttime sleep of less than 5 h (β = 0.049) and more than 9 h of sleep overall (β = 0.124) were linked to a higher risk of T2DM. Similarly, in the U.S. population, sleeping less than 5 h (β = 0.056), 5–6 h (β = 0.034), and more than 8 h (β = 0.052) also significantly increased the risk of T2DM. Moreover, sleeping in later than 1:00 a.m. or before 10:00 p.m. raises the chance of acquiring T2DM. A correlation between the incidence of T2DM and increased connectivity in the default mode network (DMN), visual network (VN), central executive network, (CEN), and motor networks (MN) was found by MR analysis. Additionally, mediation analysis revealed that sleep disorders, naps during the day, and daytime sleep affect T2DM morbidity via changing connectivity in the central executive, motor, and default mode networks. Conclusions According to our research, changed brain functional network connectivity and sleep habits, significantly influence the risk of T2DM. This demonstrates the critical function that brain networks play in modulating the link between sleep patterns and the prevalence of T2DM.https://doi.org/10.1186/s13098-025-01777-7Sleep habitsBrain functional networksType 2 diabetes mellitusCohort studyMendelian randomization
spellingShingle Faquan Hu
Qian Gong
Liyuan Xiong
Wei Zhao
Lingxiu Li
Yujiao Zheng
Sleep habits, brain functional networks, and type 2 diabetes risk: cohort studies from North American and Asian populations and mediating Mendelian randomization analyses
Diabetology & Metabolic Syndrome
Sleep habits
Brain functional networks
Type 2 diabetes mellitus
Cohort study
Mendelian randomization
title Sleep habits, brain functional networks, and type 2 diabetes risk: cohort studies from North American and Asian populations and mediating Mendelian randomization analyses
title_full Sleep habits, brain functional networks, and type 2 diabetes risk: cohort studies from North American and Asian populations and mediating Mendelian randomization analyses
title_fullStr Sleep habits, brain functional networks, and type 2 diabetes risk: cohort studies from North American and Asian populations and mediating Mendelian randomization analyses
title_full_unstemmed Sleep habits, brain functional networks, and type 2 diabetes risk: cohort studies from North American and Asian populations and mediating Mendelian randomization analyses
title_short Sleep habits, brain functional networks, and type 2 diabetes risk: cohort studies from North American and Asian populations and mediating Mendelian randomization analyses
title_sort sleep habits brain functional networks and type 2 diabetes risk cohort studies from north american and asian populations and mediating mendelian randomization analyses
topic Sleep habits
Brain functional networks
Type 2 diabetes mellitus
Cohort study
Mendelian randomization
url https://doi.org/10.1186/s13098-025-01777-7
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