Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet

Obesity is associated with numerous metabolic disorders, and dietary polyphenols have been confirmed to have beneficial effects on the metabolism in obesity. However, the effect of 3-(3′,4′-dihydroxyphenyl)propanoic acid (DHPA) and 3′,4′-dihydroxyphenylacetic acid (DHAA), two main metabolites of die...

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Main Authors: Wanbing Chen, Ruonan Liu, Xiaoling Zhu, Qun Lu, Hong Yang, Rui Liu
Format: Article
Language:English
Published: Tsinghua University Press 2024-01-01
Series:Food Science and Human Wellness
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Online Access:http://www.sciencedirect.com/science/article/pii/S2213453023001428
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author Wanbing Chen
Ruonan Liu
Xiaoling Zhu
Qun Lu
Hong Yang
Rui Liu
author_facet Wanbing Chen
Ruonan Liu
Xiaoling Zhu
Qun Lu
Hong Yang
Rui Liu
author_sort Wanbing Chen
collection DOAJ
description Obesity is associated with numerous metabolic disorders, and dietary polyphenols have been confirmed to have beneficial effects on the metabolism in obesity. However, the effect of 3-(3′,4′-dihydroxyphenyl)propanoic acid (DHPA) and 3′,4′-dihydroxyphenylacetic acid (DHAA), two main metabolites of dietary polyphenols, on obesity remains poorly understood. In this study, DHPA and DHAA were found to alleviate obesity, as well as regulate insulin resistance, lipid metabolism, and oxidative stress response in high-fat diet (HFD) mice. Surprisingly, the 16S rRNA sequencing and UHPLC-Q-TOF/MS demonstrated that DHPA and DHAA only slightly disturbed the intestinal microbiome, but significantly altered the urine metabolome of HFD mice mainly by regulating pentose and glucuronate interconversion, tyrosine metabolism, pentose phosphate and tricarboxylic acid (TCA) cycle as indicated by metabolic pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Correlation analysis revealed that the differential metabolites are strongly associated with body weight, blood glucose, insulin level, and superoxide dismutase (SOD) enzyme activity. Our results revealed that DHPA and DHAA exert their anti-obesity effect by regulating important metabolites in the glucose, lipid and tyrosine metabolism pathways.
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publishDate 2024-01-01
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series Food Science and Human Wellness
spelling doaj-art-c1893725c48d424c8f2f650a2a52b48c2025-02-03T06:57:18ZengTsinghua University PressFood Science and Human Wellness2213-45302024-01-01131327338Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat dietWanbing Chen0Ruonan Liu1Xiaoling Zhu2Qun Lu3Hong Yang4Rui Liu5College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of education, Wuhan 430000, China; Wuhan Engineering Research Center of Bee Products on Quality and Safety Control, Wuhan 430000, China; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan 430000, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of education, Wuhan 430000, China; Wuhan Engineering Research Center of Bee Products on Quality and Safety Control, Wuhan 430000, China; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan 430000, ChinaHubei Provincial Institute for Food Supervision and Test, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of education, Wuhan 430000, China; Wuhan Engineering Research Center of Bee Products on Quality and Safety Control, Wuhan 430000, China; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan 430000, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of education, Wuhan 430000, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of education, Wuhan 430000, China; Wuhan Engineering Research Center of Bee Products on Quality and Safety Control, Wuhan 430000, China; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan 430000, China; Corresponding author at: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China.Obesity is associated with numerous metabolic disorders, and dietary polyphenols have been confirmed to have beneficial effects on the metabolism in obesity. However, the effect of 3-(3′,4′-dihydroxyphenyl)propanoic acid (DHPA) and 3′,4′-dihydroxyphenylacetic acid (DHAA), two main metabolites of dietary polyphenols, on obesity remains poorly understood. In this study, DHPA and DHAA were found to alleviate obesity, as well as regulate insulin resistance, lipid metabolism, and oxidative stress response in high-fat diet (HFD) mice. Surprisingly, the 16S rRNA sequencing and UHPLC-Q-TOF/MS demonstrated that DHPA and DHAA only slightly disturbed the intestinal microbiome, but significantly altered the urine metabolome of HFD mice mainly by regulating pentose and glucuronate interconversion, tyrosine metabolism, pentose phosphate and tricarboxylic acid (TCA) cycle as indicated by metabolic pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Correlation analysis revealed that the differential metabolites are strongly associated with body weight, blood glucose, insulin level, and superoxide dismutase (SOD) enzyme activity. Our results revealed that DHPA and DHAA exert their anti-obesity effect by regulating important metabolites in the glucose, lipid and tyrosine metabolism pathways.http://www.sciencedirect.com/science/article/pii/S2213453023001428ObesityDietary polyphenol metabolitesGut microbiotaMetabonomics
spellingShingle Wanbing Chen
Ruonan Liu
Xiaoling Zhu
Qun Lu
Hong Yang
Rui Liu
Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet
Food Science and Human Wellness
Obesity
Dietary polyphenol metabolites
Gut microbiota
Metabonomics
title Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet
title_full Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet
title_fullStr Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet
title_full_unstemmed Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet
title_short Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet
title_sort microbial phenolic metabolites 3 3 4 dihydroxyphenyl propanoic acid and 3 4 dihydroxyphenylacetic acid prevent obesity in mice fed with high fat diet
topic Obesity
Dietary polyphenol metabolites
Gut microbiota
Metabonomics
url http://www.sciencedirect.com/science/article/pii/S2213453023001428
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