Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet
Obesity is associated with numerous metabolic disorders, and dietary polyphenols have been confirmed to have beneficial effects on the metabolism in obesity. However, the effect of 3-(3′,4′-dihydroxyphenyl)propanoic acid (DHPA) and 3′,4′-dihydroxyphenylacetic acid (DHAA), two main metabolites of die...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2024-01-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023001428 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546424242307072 |
|---|---|
| author | Wanbing Chen Ruonan Liu Xiaoling Zhu Qun Lu Hong Yang Rui Liu |
| author_facet | Wanbing Chen Ruonan Liu Xiaoling Zhu Qun Lu Hong Yang Rui Liu |
| author_sort | Wanbing Chen |
| collection | DOAJ |
| description | Obesity is associated with numerous metabolic disorders, and dietary polyphenols have been confirmed to have beneficial effects on the metabolism in obesity. However, the effect of 3-(3′,4′-dihydroxyphenyl)propanoic acid (DHPA) and 3′,4′-dihydroxyphenylacetic acid (DHAA), two main metabolites of dietary polyphenols, on obesity remains poorly understood. In this study, DHPA and DHAA were found to alleviate obesity, as well as regulate insulin resistance, lipid metabolism, and oxidative stress response in high-fat diet (HFD) mice. Surprisingly, the 16S rRNA sequencing and UHPLC-Q-TOF/MS demonstrated that DHPA and DHAA only slightly disturbed the intestinal microbiome, but significantly altered the urine metabolome of HFD mice mainly by regulating pentose and glucuronate interconversion, tyrosine metabolism, pentose phosphate and tricarboxylic acid (TCA) cycle as indicated by metabolic pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Correlation analysis revealed that the differential metabolites are strongly associated with body weight, blood glucose, insulin level, and superoxide dismutase (SOD) enzyme activity. Our results revealed that DHPA and DHAA exert their anti-obesity effect by regulating important metabolites in the glucose, lipid and tyrosine metabolism pathways. |
| format | Article |
| id | doaj-art-c1893725c48d424c8f2f650a2a52b48c |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-c1893725c48d424c8f2f650a2a52b48c2025-02-03T06:57:18ZengTsinghua University PressFood Science and Human Wellness2213-45302024-01-01131327338Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat dietWanbing Chen0Ruonan Liu1Xiaoling Zhu2Qun Lu3Hong Yang4Rui Liu5College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of education, Wuhan 430000, China; Wuhan Engineering Research Center of Bee Products on Quality and Safety Control, Wuhan 430000, China; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan 430000, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of education, Wuhan 430000, China; Wuhan Engineering Research Center of Bee Products on Quality and Safety Control, Wuhan 430000, China; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan 430000, ChinaHubei Provincial Institute for Food Supervision and Test, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of education, Wuhan 430000, China; Wuhan Engineering Research Center of Bee Products on Quality and Safety Control, Wuhan 430000, China; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan 430000, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of education, Wuhan 430000, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of education, Wuhan 430000, China; Wuhan Engineering Research Center of Bee Products on Quality and Safety Control, Wuhan 430000, China; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan 430000, China; Corresponding author at: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China.Obesity is associated with numerous metabolic disorders, and dietary polyphenols have been confirmed to have beneficial effects on the metabolism in obesity. However, the effect of 3-(3′,4′-dihydroxyphenyl)propanoic acid (DHPA) and 3′,4′-dihydroxyphenylacetic acid (DHAA), two main metabolites of dietary polyphenols, on obesity remains poorly understood. In this study, DHPA and DHAA were found to alleviate obesity, as well as regulate insulin resistance, lipid metabolism, and oxidative stress response in high-fat diet (HFD) mice. Surprisingly, the 16S rRNA sequencing and UHPLC-Q-TOF/MS demonstrated that DHPA and DHAA only slightly disturbed the intestinal microbiome, but significantly altered the urine metabolome of HFD mice mainly by regulating pentose and glucuronate interconversion, tyrosine metabolism, pentose phosphate and tricarboxylic acid (TCA) cycle as indicated by metabolic pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Correlation analysis revealed that the differential metabolites are strongly associated with body weight, blood glucose, insulin level, and superoxide dismutase (SOD) enzyme activity. Our results revealed that DHPA and DHAA exert their anti-obesity effect by regulating important metabolites in the glucose, lipid and tyrosine metabolism pathways.http://www.sciencedirect.com/science/article/pii/S2213453023001428ObesityDietary polyphenol metabolitesGut microbiotaMetabonomics |
| spellingShingle | Wanbing Chen Ruonan Liu Xiaoling Zhu Qun Lu Hong Yang Rui Liu Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet Food Science and Human Wellness Obesity Dietary polyphenol metabolites Gut microbiota Metabonomics |
| title | Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet |
| title_full | Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet |
| title_fullStr | Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet |
| title_full_unstemmed | Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet |
| title_short | Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet |
| title_sort | microbial phenolic metabolites 3 3 4 dihydroxyphenyl propanoic acid and 3 4 dihydroxyphenylacetic acid prevent obesity in mice fed with high fat diet |
| topic | Obesity Dietary polyphenol metabolites Gut microbiota Metabonomics |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023001428 |
| work_keys_str_mv | AT wanbingchen microbialphenolicmetabolites334dihydroxyphenylpropanoicacidand34dihydroxyphenylaceticacidpreventobesityinmicefedwithhighfatdiet AT ruonanliu microbialphenolicmetabolites334dihydroxyphenylpropanoicacidand34dihydroxyphenylaceticacidpreventobesityinmicefedwithhighfatdiet AT xiaolingzhu microbialphenolicmetabolites334dihydroxyphenylpropanoicacidand34dihydroxyphenylaceticacidpreventobesityinmicefedwithhighfatdiet AT qunlu microbialphenolicmetabolites334dihydroxyphenylpropanoicacidand34dihydroxyphenylaceticacidpreventobesityinmicefedwithhighfatdiet AT hongyang microbialphenolicmetabolites334dihydroxyphenylpropanoicacidand34dihydroxyphenylaceticacidpreventobesityinmicefedwithhighfatdiet AT ruiliu microbialphenolicmetabolites334dihydroxyphenylpropanoicacidand34dihydroxyphenylaceticacidpreventobesityinmicefedwithhighfatdiet |