Hybrid material of sustainable newspaper waste-derived cellulose for histamine sensing in kombucha tea
This study introduces a novel approach for sensing histamine by developing sensors from cellulose-based newspaper waste. The cellulose-based sensors, featuring diamine oxidase (DAO) as the sensing agent in cooperation with horseradish peroxidase (HRP) and 3,3′,5,5′-tetramethylbenzidine (TMB) solutio...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-05-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525004250 |
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| Summary: | This study introduces a novel approach for sensing histamine by developing sensors from cellulose-based newspaper waste. The cellulose-based sensors, featuring diamine oxidase (DAO) as the sensing agent in cooperation with horseradish peroxidase (HRP) and 3,3′,5,5′-tetramethylbenzidine (TMB) solutions, are designed and assembled for targeted histamine detection. Experimental results demonstrate the sensor's exceptional sensitivity, with the observed color change serving as a reliable indicator of histamine concentration. In the presence of peroxidase and H2O2, the hydroxypropyl methylcellulose (HPMC)/DAO-immobilized enzyme-hydrolyzed cellulose-based film sensor gets oxidized, resulting in a blue spot. The highest sensitivity to histamine (3.30 mg/mL) was exhibited by the 3 w/w % HPMC/DAO-immobilized enzyme-hydrolyzed cellulose-based film, which showed the lowest b* value (−8.85 ± 0.01). This study showcases the potential of these films as an effective and cost-efficient platform for histamine detection in fermented beverages, offering promising applications in food safety screening. Ultimately, the research underscores the environmental sustainability of the HPMC-based hybrid material. |
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| ISSN: | 2590-1575 |