OPTIMIZATION OF ANNEALING TEMPERATURE AND PRIMER CONCENTRATION OF CYTOCHROME B (CYT B) GENE FOR PIG DNA DETECTION WITH REAL-TIME PCR METHOD

Background: One company in Indonesia has developed a pig DNA detection kit by designing primers with the real-time Polymerase Chain Reaction (PCR) method using the cytochrome b (cyt b) gene. It is necessary to optimize the PCR process to optimize pig DNA detection, including annealing temperature an...

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Bibliographic Details
Main Authors: Novia Azahra Fatoni, Ai Djuminar, Acep Tantan Hardiana, Fusvita Merdekawati
Format: Article
Language:English
Published: Universitas Airlangga 2025-03-01
Series:Journal of Vocational Health Studies
Subjects:
Online Access:https://e-journal.unair.ac.id/JVHS/article/view/48339
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