Pharmacokinetics, pharmacogenetics, and toxicity of co-administered efavirenz and isoniazid

Background: CYP2B6 slow metabolisers have higher efavirenz concentrations, which are further increased by isoniazid inhibiting efavirenz’s accessory metabolic pathway. Objectives: We investigated the association between CYP2B6 genotype and toxicity in people living with HIV (PLWH) on isoniazid and...

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Main Authors: Jessica Taylor, Gary Maartens, Simiso Sokhela, Nomathemba Chandiwana, Godspower Akpomiemie, Francois Venter, Phumla Sinxadi
Format: Article
Language:English
Published: AOSIS 2025-03-01
Series:Southern African Journal of HIV Medicine
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Online Access:https://sajhivmed.org.za/index.php/hivmed/article/view/1661
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author Jessica Taylor
Gary Maartens
Simiso Sokhela
Nomathemba Chandiwana
Godspower Akpomiemie
Francois Venter
Phumla Sinxadi
author_facet Jessica Taylor
Gary Maartens
Simiso Sokhela
Nomathemba Chandiwana
Godspower Akpomiemie
Francois Venter
Phumla Sinxadi
author_sort Jessica Taylor
collection DOAJ
description Background: CYP2B6 slow metabolisers have higher efavirenz concentrations, which are further increased by isoniazid inhibiting efavirenz’s accessory metabolic pathway. Objectives: We investigated the association between CYP2B6 genotype and toxicity in people living with HIV (PLWH) on isoniazid and efavirenz. Method: We enrolled participants from the efavirenz arm of the ADVANCE trial (reference no.: NCT03122262), who received isoniazid and consented to genotyping. We compared efavirenz concentrations on and off isoniazid, stratified by CYP2B6 genotype. We explored associations between the CYP2B6 genotype and efavirenz concentrations on isoniazid; and changes over 24 weeks in lipids, alanine aminotransferase (ALT), fasting plasma glucose (FPG), sleep quality, and Modified Mini Screen (MMS) scores. Results: A total of 168 participants, median age 31 years, 57% female, had classifiable CYP2B6 genotypes. Efavirenz concentrations on isoniazid were higher (pseudo-median difference 0.49 µg/mL (95% confidence interval [CI] [0.19–0.91]) and associated with increases in total and high-density lipoprotein (HDL)-cholesterol. CYP2B6 slow metabolisers had higher efavirenz concentrations on isoniazid than extensive metabolisers (β = 1.66 [95% CI 0.98–2.34]). CYP2B6 slow metabolisers had greater increases in total (β = 0.44 mmol/L [95% CI 0.01–0.86]) and HDL-cholesterol (β = 0.39 mmol/L [95% CI 0.21–0.57]) than extensive metabolisers. There were no associations between efavirenz concentrations or CYP2B6 genotype, and change in ALT, FPG, low-density lipoprotein (LDL)-cholesterol, triglycerides, sleep quality, or MMS scores. Conclusion: CYP2B6 slow metabolisers on isoniazid and efavirenz had greater efavirenz concentrations and increases in total and HDL-cholesterol. We found no association between CYP2B6 genotype or efavirenz concentrations and sleep or psychiatric symptoms.
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series Southern African Journal of HIV Medicine
spelling doaj-art-bf06b57596ec4b24b52697500111b2482025-08-20T03:06:40ZengAOSISSouthern African Journal of HIV Medicine1608-96932078-67512025-03-01261e1e1110.4102/sajhivmed.v26i1.1661893Pharmacokinetics, pharmacogenetics, and toxicity of co-administered efavirenz and isoniazidJessica Taylor0Gary Maartens1Simiso Sokhela2Nomathemba Chandiwana3Godspower Akpomiemie4Francois Venter5Phumla Sinxadi6Division of Clinical Pharmacology, Department of Medicine, Faculty of Health Sciences, University of Cape Town, Cape TownDivision of Clinical Pharmacology, Department of Medicine, Faculty of Health Sciences, University of Cape Town, Cape TownEzintsha, Wits Health Consortium, Faculty of Health Sciences, University of the Witwatersrand, JohannesburgEzintsha, Wits Health Consortium, Faculty of Health Sciences, University of the Witwatersrand, JohannesburgEzintsha, Wits Health Consortium, Faculty of Health Sciences, University of the Witwatersrand, JohannesburgEzintsha, Wits Health Consortium, Faculty of Health Sciences, University of the Witwatersrand, JohannesburgDivision of Clinical Pharmacology, Department of Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa; and, SAMRC/UCT Platform for Pharmacogenomics Research and Translation, South African Medical Research Council, Cape TownBackground: CYP2B6 slow metabolisers have higher efavirenz concentrations, which are further increased by isoniazid inhibiting efavirenz’s accessory metabolic pathway. Objectives: We investigated the association between CYP2B6 genotype and toxicity in people living with HIV (PLWH) on isoniazid and efavirenz. Method: We enrolled participants from the efavirenz arm of the ADVANCE trial (reference no.: NCT03122262), who received isoniazid and consented to genotyping. We compared efavirenz concentrations on and off isoniazid, stratified by CYP2B6 genotype. We explored associations between the CYP2B6 genotype and efavirenz concentrations on isoniazid; and changes over 24 weeks in lipids, alanine aminotransferase (ALT), fasting plasma glucose (FPG), sleep quality, and Modified Mini Screen (MMS) scores. Results: A total of 168 participants, median age 31 years, 57% female, had classifiable CYP2B6 genotypes. Efavirenz concentrations on isoniazid were higher (pseudo-median difference 0.49 µg/mL (95% confidence interval [CI] [0.19–0.91]) and associated with increases in total and high-density lipoprotein (HDL)-cholesterol. CYP2B6 slow metabolisers had higher efavirenz concentrations on isoniazid than extensive metabolisers (β = 1.66 [95% CI 0.98–2.34]). CYP2B6 slow metabolisers had greater increases in total (β = 0.44 mmol/L [95% CI 0.01–0.86]) and HDL-cholesterol (β = 0.39 mmol/L [95% CI 0.21–0.57]) than extensive metabolisers. There were no associations between efavirenz concentrations or CYP2B6 genotype, and change in ALT, FPG, low-density lipoprotein (LDL)-cholesterol, triglycerides, sleep quality, or MMS scores. Conclusion: CYP2B6 slow metabolisers on isoniazid and efavirenz had greater efavirenz concentrations and increases in total and HDL-cholesterol. We found no association between CYP2B6 genotype or efavirenz concentrations and sleep or psychiatric symptoms.https://sajhivmed.org.za/index.php/hivmed/article/view/1661hivisoniazidefavirenzcyp2b6neurotoxicity.
spellingShingle Jessica Taylor
Gary Maartens
Simiso Sokhela
Nomathemba Chandiwana
Godspower Akpomiemie
Francois Venter
Phumla Sinxadi
Pharmacokinetics, pharmacogenetics, and toxicity of co-administered efavirenz and isoniazid
Southern African Journal of HIV Medicine
hiv
isoniazid
efavirenz
cyp2b6
neurotoxicity.
title Pharmacokinetics, pharmacogenetics, and toxicity of co-administered efavirenz and isoniazid
title_full Pharmacokinetics, pharmacogenetics, and toxicity of co-administered efavirenz and isoniazid
title_fullStr Pharmacokinetics, pharmacogenetics, and toxicity of co-administered efavirenz and isoniazid
title_full_unstemmed Pharmacokinetics, pharmacogenetics, and toxicity of co-administered efavirenz and isoniazid
title_short Pharmacokinetics, pharmacogenetics, and toxicity of co-administered efavirenz and isoniazid
title_sort pharmacokinetics pharmacogenetics and toxicity of co administered efavirenz and isoniazid
topic hiv
isoniazid
efavirenz
cyp2b6
neurotoxicity.
url https://sajhivmed.org.za/index.php/hivmed/article/view/1661
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