Detection of Single-Copy Sequences with Digoxigenin-Labeled Probes in a Complex Plant Genome After Separation on Pulsed-Field Gels
In recent years, the application of rare cutting restriction enzymes, the separation of resulting DNA fragments on pulsed-field gels and the subsequent Southern blot analysis using radioactively labeled probes have been standard laboratory methods to create long-range physical maps of complex genome...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-12-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96216st04 |
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