Osteoarthritis-like changes in rat temporomandibular joint induced by unilateral anterior large overjet treatment
Abstract Temporomandibular joint osteoarthritis (TMJOA) is a common degenerative disease that causes chronic pain and joint dysfunction. However, the current understanding of TMJOA pathogenesis is limited and necessitates further research. Animal models are crucial for investigating TMJOA due to the...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-81306-0 |
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| author | Xinru Xie Rui Chao Yi Mao Tianhao Wan Yexin Wang Yan Zhu Weifeng Xu Xuzhuo Chen Yong Wang Zhigui Ma Shanyong Zhang |
| author_facet | Xinru Xie Rui Chao Yi Mao Tianhao Wan Yexin Wang Yan Zhu Weifeng Xu Xuzhuo Chen Yong Wang Zhigui Ma Shanyong Zhang |
| author_sort | Xinru Xie |
| collection | DOAJ |
| description | Abstract Temporomandibular joint osteoarthritis (TMJOA) is a common degenerative disease that causes chronic pain and joint dysfunction. However, the current understanding of TMJOA pathogenesis is limited and necessitates further research. Animal models are crucial for investigating TMJOA due to the scarcity of clinical samples. Class II malocclusion is an occlusal type highly associated with TMJOA, but it currently lacks appropriate animal models for simulating this malocclusion in research. Therefore, this study develops a new malocclusion model using a unilateral anterior large overjet (UALO) dental device to cause Class II malocclusion characteristics and TMJOA-like pathological alterations in rats. By inducing a posteriorly positioned condyle, the UALO device effectively results in cartilage degradation, subchondral bone loss, condylar volume reduction, and mandibular retrusion. Furthermore, RNA sequencing of condylar cartilages revealed that the oxidative stress of chondrocytes was elevated under the UALO-triggered abnormal mechanical stress. Disruption of antioxidant systems and mitochondrial dysfunction are involved in cartilage degeneration. The current study provides a novel and reliable rat model suitable for TMJOA research and offers insights into the disease’s potential mechanistic pathways and molecular targets, contributing to a deeper understanding of TMJOA. |
| format | Article |
| id | doaj-art-be3fb53b22eb4fce874176451d9d1df8 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-be3fb53b22eb4fce874176451d9d1df82025-01-12T12:19:22ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-024-81306-0Osteoarthritis-like changes in rat temporomandibular joint induced by unilateral anterior large overjet treatmentXinru Xie0Rui Chao1Yi Mao2Tianhao Wan3Yexin Wang4Yan Zhu5Weifeng Xu6Xuzhuo Chen7Yong Wang8Zhigui Ma9Shanyong Zhang10Department of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineState Key Laboratory of Oral Diseases, Department of Orthognathic and TMJ Surgery, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan UniversityDepartment of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Oral Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineAbstract Temporomandibular joint osteoarthritis (TMJOA) is a common degenerative disease that causes chronic pain and joint dysfunction. However, the current understanding of TMJOA pathogenesis is limited and necessitates further research. Animal models are crucial for investigating TMJOA due to the scarcity of clinical samples. Class II malocclusion is an occlusal type highly associated with TMJOA, but it currently lacks appropriate animal models for simulating this malocclusion in research. Therefore, this study develops a new malocclusion model using a unilateral anterior large overjet (UALO) dental device to cause Class II malocclusion characteristics and TMJOA-like pathological alterations in rats. By inducing a posteriorly positioned condyle, the UALO device effectively results in cartilage degradation, subchondral bone loss, condylar volume reduction, and mandibular retrusion. Furthermore, RNA sequencing of condylar cartilages revealed that the oxidative stress of chondrocytes was elevated under the UALO-triggered abnormal mechanical stress. Disruption of antioxidant systems and mitochondrial dysfunction are involved in cartilage degeneration. The current study provides a novel and reliable rat model suitable for TMJOA research and offers insights into the disease’s potential mechanistic pathways and molecular targets, contributing to a deeper understanding of TMJOA.https://doi.org/10.1038/s41598-024-81306-0Temporomandibular joint osteoarthritisCartilage degenerationAbnormal mechanical stressMandibular retrusionOxidative stressMitochondrial dysfunction |
| spellingShingle | Xinru Xie Rui Chao Yi Mao Tianhao Wan Yexin Wang Yan Zhu Weifeng Xu Xuzhuo Chen Yong Wang Zhigui Ma Shanyong Zhang Osteoarthritis-like changes in rat temporomandibular joint induced by unilateral anterior large overjet treatment Scientific Reports Temporomandibular joint osteoarthritis Cartilage degeneration Abnormal mechanical stress Mandibular retrusion Oxidative stress Mitochondrial dysfunction |
| title | Osteoarthritis-like changes in rat temporomandibular joint induced by unilateral anterior large overjet treatment |
| title_full | Osteoarthritis-like changes in rat temporomandibular joint induced by unilateral anterior large overjet treatment |
| title_fullStr | Osteoarthritis-like changes in rat temporomandibular joint induced by unilateral anterior large overjet treatment |
| title_full_unstemmed | Osteoarthritis-like changes in rat temporomandibular joint induced by unilateral anterior large overjet treatment |
| title_short | Osteoarthritis-like changes in rat temporomandibular joint induced by unilateral anterior large overjet treatment |
| title_sort | osteoarthritis like changes in rat temporomandibular joint induced by unilateral anterior large overjet treatment |
| topic | Temporomandibular joint osteoarthritis Cartilage degeneration Abnormal mechanical stress Mandibular retrusion Oxidative stress Mitochondrial dysfunction |
| url | https://doi.org/10.1038/s41598-024-81306-0 |
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