Fisetin limits Chikungunya virus-induced apoptosis hallmarks in hepatocellular carcinoma cells
Abstract The study investigated the protective effects of fisetin on chikungunya virus (CHIKV)-induced apoptosis hallmarks in Huh7 cells. Fisetin significantly reduced CHIKV RNA levels and viral infectivity, outperforming Z-VAD-FMK and cisplatin. At 30 µM, fisetin markedly decreased (by > 90%) th...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-09213-6 |
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| author | Rafidah Lani Pouya Hassandarvish Sazaly AbuBakar |
| author_facet | Rafidah Lani Pouya Hassandarvish Sazaly AbuBakar |
| author_sort | Rafidah Lani |
| collection | DOAJ |
| description | Abstract The study investigated the protective effects of fisetin on chikungunya virus (CHIKV)-induced apoptosis hallmarks in Huh7 cells. Fisetin significantly reduced CHIKV RNA levels and viral infectivity, outperforming Z-VAD-FMK and cisplatin. At 30 µM, fisetin markedly decreased (by > 90%) the number of infectious viral particles at 24 and 48 h post-infection (hpi). Fisetin also hindered CHIKV-induced DNA fragmentation, with the lowest levels observed in CHIKV-infected cells treated with fisetin compared to other treatments. Immunoblot analysis revealed that fisetin inhibited caspase-mediated PARP cleavage and significantly reduced cleaved PARP levels, indicating decreased apoptosis. Additionally, fisetin diminished the expression of cleaved caspase-3 and HSP-27 proteins, while restoring HIF-1α protein levels, suggesting a protective role against CHIKV-induced apoptosis. The study highlights the potential of fisetin as an effective antiviral agent against CHIKV through the modulation of apoptosis and oxidative stress pathways. These findings underscore the therapeutic promise of fisetin for treating CHIKV-induced apoptosis and warrant further investigation to explore its clinical applications and optimize its use in antiviral therapy. |
| format | Article |
| id | doaj-art-bceb9fccf80f43419305f0951e01f870 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-bceb9fccf80f43419305f0951e01f8702025-08-20T04:03:01ZengNature PortfolioScientific Reports2045-23222025-07-0115111010.1038/s41598-025-09213-6Fisetin limits Chikungunya virus-induced apoptosis hallmarks in hepatocellular carcinoma cellsRafidah Lani0Pouya Hassandarvish1Sazaly AbuBakar2Department of Medical Microbiology, Faculty of Medicine, Universiti MalayaTropical Infectious Diseases Research and Education Centre (TIDREC), Universiti MalayaTropical Infectious Diseases Research and Education Centre (TIDREC), Universiti MalayaAbstract The study investigated the protective effects of fisetin on chikungunya virus (CHIKV)-induced apoptosis hallmarks in Huh7 cells. Fisetin significantly reduced CHIKV RNA levels and viral infectivity, outperforming Z-VAD-FMK and cisplatin. At 30 µM, fisetin markedly decreased (by > 90%) the number of infectious viral particles at 24 and 48 h post-infection (hpi). Fisetin also hindered CHIKV-induced DNA fragmentation, with the lowest levels observed in CHIKV-infected cells treated with fisetin compared to other treatments. Immunoblot analysis revealed that fisetin inhibited caspase-mediated PARP cleavage and significantly reduced cleaved PARP levels, indicating decreased apoptosis. Additionally, fisetin diminished the expression of cleaved caspase-3 and HSP-27 proteins, while restoring HIF-1α protein levels, suggesting a protective role against CHIKV-induced apoptosis. The study highlights the potential of fisetin as an effective antiviral agent against CHIKV through the modulation of apoptosis and oxidative stress pathways. These findings underscore the therapeutic promise of fisetin for treating CHIKV-induced apoptosis and warrant further investigation to explore its clinical applications and optimize its use in antiviral therapy.https://doi.org/10.1038/s41598-025-09213-6ApoptosisChikungunyaFlavonoidsFisetinAntiviralCytoprotective |
| spellingShingle | Rafidah Lani Pouya Hassandarvish Sazaly AbuBakar Fisetin limits Chikungunya virus-induced apoptosis hallmarks in hepatocellular carcinoma cells Scientific Reports Apoptosis Chikungunya Flavonoids Fisetin Antiviral Cytoprotective |
| title | Fisetin limits Chikungunya virus-induced apoptosis hallmarks in hepatocellular carcinoma cells |
| title_full | Fisetin limits Chikungunya virus-induced apoptosis hallmarks in hepatocellular carcinoma cells |
| title_fullStr | Fisetin limits Chikungunya virus-induced apoptosis hallmarks in hepatocellular carcinoma cells |
| title_full_unstemmed | Fisetin limits Chikungunya virus-induced apoptosis hallmarks in hepatocellular carcinoma cells |
| title_short | Fisetin limits Chikungunya virus-induced apoptosis hallmarks in hepatocellular carcinoma cells |
| title_sort | fisetin limits chikungunya virus induced apoptosis hallmarks in hepatocellular carcinoma cells |
| topic | Apoptosis Chikungunya Flavonoids Fisetin Antiviral Cytoprotective |
| url | https://doi.org/10.1038/s41598-025-09213-6 |
| work_keys_str_mv | AT rafidahlani fisetinlimitschikungunyavirusinducedapoptosishallmarksinhepatocellularcarcinomacells AT pouyahassandarvish fisetinlimitschikungunyavirusinducedapoptosishallmarksinhepatocellularcarcinomacells AT sazalyabubakar fisetinlimitschikungunyavirusinducedapoptosishallmarksinhepatocellularcarcinomacells |