Transcriptome Analysis of <i>Sclerotium rolfsii</i>: Unraveling Impact of Glycolytic Pathway on Substrate Utilization and Microbial Polysaccharide Production

Transcriptome Analysis of <i>Sclerotium rolfsii</i>: Unraveling Impact of Glycolytic Pathway on Substrate Utilization and Microbial Polysaccharide Production

Scleroglucan is the extracellular polysaccharide (EPS) produced by <i>Sclerotium rolfsii</i> (<i>S. rolfsii</i>). The low EPS titer and limited substrate utilization of <i>S. rolfsii</i> present significant challenges in the fermentation process, restricting indus...

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Bibliographic Details
Main Authors: Jia Song, Junfeng Li, Chenrui Zhen, Juan Du, Rui Zhao, Bingqian Fan, Jiayi Hou, Bingning Gao, Yu Zheng, Linna Tu, Min Wang
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Fermentation
Subjects:
biosynthetic processes
<i>Sclerotium rolfsii</i>
different carbon sources
transcriptome analysis
substrate utilization
precursor addition
Online Access:https://www.mdpi.com/2311-5637/11/3/143
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Summary:Scleroglucan is the extracellular polysaccharide (EPS) produced by <i>Sclerotium rolfsii</i> (<i>S. rolfsii</i>). The low EPS titer and limited substrate utilization of <i>S. rolfsii</i> present significant challenges in the fermentation process, restricting industrial applications of scleroglucan. In this study, we performed a transcriptomic analysis on the mycelium of <i>S. rolfsii</i> fermented with different carbon sources. The key genes involved in polysaccharide biosynthesis (6-phosphofructokinase 1 (<i>PFK1</i>), pyruvate decarboxylase (<i>PDC</i>), aldehyde dehydrogenase (NAD (P)+) (<i>ALDH3</i>), and acetyl-CoA synthase (<i>ACS</i>)) were identified and their roles in the process were investigated. The supplementation of specific precursors—fructose-6-phosphate, pyruvate, aldehydes, and acetate—was shown to enhance both the polysaccharide titer and substrate utilization. By adding precursors, the titer of SEPS produced in a 5 L fermentation tank reached 48.69 ± 3.8 g/L. Notably, the addition of these precursors increased the titer of EPS fermented with sucrose (SEPS) by 65.63% and substrate utilization by 119.3%, while the titer of EPS fermented with lactose (LEPS) rose by 80.29% and substrate utilization rose by 47.08%. These findings suggest that precursor supplementation can effectively improve polysaccharide production and substrate efficiency, thereby minimizing resource waste and environmental impact.
ISSN:2311-5637

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