The role of renal and liver function in clinical ctDNA testing.

Circulating tumor DNA (ctDNA) has high clinical potential in early cancer detection. The renal system and the liver are involved in clearing circulating cell free DNA (cfDNA) from the blood. Recent studies on mice show that inhibiting the liver's ability to clear cfDNA results in elevated ctDNA...

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Main Authors: Jens Bo Koudahl Conrad, Tenna Vesterman Henriksen, Jesper Berg Nors, Mads Heilskov Rasmussen, Mai-Britt Worm Ørntoft, Nis Hallundbæk Schlesinger, Per Vadgaard Andersen, Kåre Andersson Gotschalck, Claus Lindbjerg Andersen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0319194
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author Jens Bo Koudahl Conrad
Tenna Vesterman Henriksen
Jesper Berg Nors
Mads Heilskov Rasmussen
Mai-Britt Worm Ørntoft
Nis Hallundbæk Schlesinger
Per Vadgaard Andersen
Kåre Andersson Gotschalck
Claus Lindbjerg Andersen
Claus Lindbjerg Andersen
author_facet Jens Bo Koudahl Conrad
Tenna Vesterman Henriksen
Jesper Berg Nors
Mads Heilskov Rasmussen
Mai-Britt Worm Ørntoft
Nis Hallundbæk Schlesinger
Per Vadgaard Andersen
Kåre Andersson Gotschalck
Claus Lindbjerg Andersen
Claus Lindbjerg Andersen
author_sort Jens Bo Koudahl Conrad
collection DOAJ
description Circulating tumor DNA (ctDNA) has high clinical potential in early cancer detection. The renal system and the liver are involved in clearing circulating cell free DNA (cfDNA) from the blood. Recent studies on mice show that inhibiting the liver's ability to clear cfDNA results in elevated ctDNA levels in blood samples. Emphasizing the need for studies in humans exploring if markers of renal and liver function are associated with cfDNA and ctDNA levels in the blood. The present study investigates if cfDNA level, ctDNA level and ctDNA detection is affected in colorectal cancer (CRC) patients with clinical biomarkers indicative of low renal and liver function. We requisitioned standard laboratory tests of renal and liver function, measured within thirty days of curative intended surgery from 846 stage I-III CRC patients. For each patient, matching preoperative cfDNA and ctDNA data was available. We investigated the correlation between impaired renal and liver function and cfDNA level, ctDNA level, and ctDNA detection. The findings revealed that variation in renal and liver function in stage I-III CRC patients did not affect cfDNA level, ctDNA level, or ctDNA detection and that ctDNA test results remain stable over a wide range of renal and liver biomarker results.
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spelling doaj-art-bc8ce730004440ee803efa365b5fdb9c2025-08-23T05:32:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e031919410.1371/journal.pone.0319194The role of renal and liver function in clinical ctDNA testing.Jens Bo Koudahl ConradTenna Vesterman HenriksenJesper Berg NorsMads Heilskov RasmussenMai-Britt Worm ØrntoftNis Hallundbæk SchlesingerPer Vadgaard AndersenKåre Andersson GotschalckClaus Lindbjerg AndersenClaus Lindbjerg AndersenCirculating tumor DNA (ctDNA) has high clinical potential in early cancer detection. The renal system and the liver are involved in clearing circulating cell free DNA (cfDNA) from the blood. Recent studies on mice show that inhibiting the liver's ability to clear cfDNA results in elevated ctDNA levels in blood samples. Emphasizing the need for studies in humans exploring if markers of renal and liver function are associated with cfDNA and ctDNA levels in the blood. The present study investigates if cfDNA level, ctDNA level and ctDNA detection is affected in colorectal cancer (CRC) patients with clinical biomarkers indicative of low renal and liver function. We requisitioned standard laboratory tests of renal and liver function, measured within thirty days of curative intended surgery from 846 stage I-III CRC patients. For each patient, matching preoperative cfDNA and ctDNA data was available. We investigated the correlation between impaired renal and liver function and cfDNA level, ctDNA level, and ctDNA detection. The findings revealed that variation in renal and liver function in stage I-III CRC patients did not affect cfDNA level, ctDNA level, or ctDNA detection and that ctDNA test results remain stable over a wide range of renal and liver biomarker results.https://doi.org/10.1371/journal.pone.0319194
spellingShingle Jens Bo Koudahl Conrad
Tenna Vesterman Henriksen
Jesper Berg Nors
Mads Heilskov Rasmussen
Mai-Britt Worm Ørntoft
Nis Hallundbæk Schlesinger
Per Vadgaard Andersen
Kåre Andersson Gotschalck
Claus Lindbjerg Andersen
Claus Lindbjerg Andersen
The role of renal and liver function in clinical ctDNA testing.
PLoS ONE
title The role of renal and liver function in clinical ctDNA testing.
title_full The role of renal and liver function in clinical ctDNA testing.
title_fullStr The role of renal and liver function in clinical ctDNA testing.
title_full_unstemmed The role of renal and liver function in clinical ctDNA testing.
title_short The role of renal and liver function in clinical ctDNA testing.
title_sort role of renal and liver function in clinical ctdna testing
url https://doi.org/10.1371/journal.pone.0319194
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