Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus)
This study aimed to evaluate the protective effects of α-tocopherol and zinc sulfate on oxidative stress parameters in ovarian granulosa cells of female Wistar rats (Rattus norvegicus) exposed to lead acetate (Pb(C₂H₃O₂)₂). A total of 25 rats were randomly divided into five groups: Control (C), lead...
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| Language: | English |
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Universitas Airlangga
2025-04-01
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| Series: | Ovozoa: Journal of Animal Reproduction |
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| Online Access: | https://e-journal.unair.ac.id/OVZ/article/view/62786 |
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| author | iqbal dwi warsito Suherni Susilowati Erma Safitri Ragil Angga Prastiya Maya Nurwartanti Yunita Amung Logam Saputro |
| author_facet | iqbal dwi warsito Suherni Susilowati Erma Safitri Ragil Angga Prastiya Maya Nurwartanti Yunita Amung Logam Saputro |
| author_sort | iqbal dwi warsito |
| collection | DOAJ |
| description | This study aimed to evaluate the protective effects of α-tocopherol and zinc sulfate on oxidative stress parameters in ovarian granulosa cells of female Wistar rats (Rattus norvegicus) exposed to lead acetate (Pb(C₂H₃O₂)₂). A total of 25 rats were randomly divided into five groups: Control (C), lead exposure only (T0), α-tocopherol + lead (T1), zinc sulfate + lead (T2), and α-tocopherol + zinc sulfate + lead (T3). Treatments were administered orally for 21 days. Superoxide dismutase (SOD) levels were measured spectrophotometrically, and p53 expression was analyzed using immunohistochemistry. The results showed that SOD levels significantly decreased in group T0 compared to the control (p <0.05), while treatment with either α-tocopherol (T1), zinc sulfate (T2), or their combination (T3) significantly improved SOD levels compared to T0. Moreover, p53 expression was markedly elevated in T0, indicating enhanced oxidative stress and potential apoptosis, whereas all antioxidant-treated groups showed reduced p53 expression, with T3 demonstrating values comparable to the control group. In conclusion, the combination of α-tocopherol and zinc sulfate provided a synergistic antioxidant effect, effectively enhancing SOD levels and suppressing p53 expression in granulosa cells of lead-exposed rats. This suggested a promising therapeutic potential of these compounds in mitigating lead-induced ovarian toxicity. |
| format | Article |
| id | doaj-art-bc666aadb3664072930d5733e049f672 |
| institution | DOAJ |
| issn | 2302-6464 2722-967X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Ovozoa: Journal of Animal Reproduction |
| spelling | doaj-art-bc666aadb3664072930d5733e049f6722025-08-20T03:18:23ZengUniversitas AirlanggaOvozoa: Journal of Animal Reproduction2302-64642722-967X2025-04-01141162410.20473/ovz.v14i1.2025.16-2460953Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus)iqbal dwi warsito0https://orcid.org/0000-0002-4010-4854Suherni Susilowati1https://orcid.org/0009-0001-5171-4914Erma Safitri2https://orcid.org/0000-0002-6817-1178Ragil Angga Prastiya3https://orcid.org/0000-0003-2658-2099Maya Nurwartanti Yunita4https://orcid.org/0000-0002-1089-0209Amung Logam Saputro5https://orcid.org/0000-0001-8132-547XInternship Veterinarian, PT. Centra Biotech Indonesia, Klaten, IndonesiaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, IndonesiaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Health, Medicine, and Life Sciences, Faculty of Health, Medicine, and Life Sciences, Universitas Airlangga, Banyuwangi, IndonesiaDepartment of Health, Medicine, and Life Sciences, Faculty of Health, Medicine, and Life Sciences, Universitas Airlangga, Banyuwangi, IndonesiaDepartment of Health, Medicine, and Life Sciences, Faculty of Health, Medicine, and Life Sciences, Universitas Airlangga, Banyuwangi, IndonesiaThis study aimed to evaluate the protective effects of α-tocopherol and zinc sulfate on oxidative stress parameters in ovarian granulosa cells of female Wistar rats (Rattus norvegicus) exposed to lead acetate (Pb(C₂H₃O₂)₂). A total of 25 rats were randomly divided into five groups: Control (C), lead exposure only (T0), α-tocopherol + lead (T1), zinc sulfate + lead (T2), and α-tocopherol + zinc sulfate + lead (T3). Treatments were administered orally for 21 days. Superoxide dismutase (SOD) levels were measured spectrophotometrically, and p53 expression was analyzed using immunohistochemistry. The results showed that SOD levels significantly decreased in group T0 compared to the control (p <0.05), while treatment with either α-tocopherol (T1), zinc sulfate (T2), or their combination (T3) significantly improved SOD levels compared to T0. Moreover, p53 expression was markedly elevated in T0, indicating enhanced oxidative stress and potential apoptosis, whereas all antioxidant-treated groups showed reduced p53 expression, with T3 demonstrating values comparable to the control group. In conclusion, the combination of α-tocopherol and zinc sulfate provided a synergistic antioxidant effect, effectively enhancing SOD levels and suppressing p53 expression in granulosa cells of lead-exposed rats. This suggested a promising therapeutic potential of these compounds in mitigating lead-induced ovarian toxicity.https://e-journal.unair.ac.id/OVZ/article/view/62786α-tocopherolgranulosa cellslead acetateoxidative stressp53zinc sulfate |
| spellingShingle | iqbal dwi warsito Suherni Susilowati Erma Safitri Ragil Angga Prastiya Maya Nurwartanti Yunita Amung Logam Saputro Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus) Ovozoa: Journal of Animal Reproduction α-tocopherol granulosa cells lead acetate oxidative stress p53 zinc sulfate |
| title | Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus) |
| title_full | Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus) |
| title_fullStr | Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus) |
| title_full_unstemmed | Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus) |
| title_short | Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus) |
| title_sort | synergistic protective effects of α tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead exposed female wistar rats rattus norvegicus |
| topic | α-tocopherol granulosa cells lead acetate oxidative stress p53 zinc sulfate |
| url | https://e-journal.unair.ac.id/OVZ/article/view/62786 |
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