In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.
Background and Objectives. A wound is one of the high-prevalence disorders that affect people’s lives physically, mentally, and financially. This study examined the Astragalus microcephalus Willd. wound healing process in in vivo and in vitro conditions by focusing on the phytoestrogen activity of t...
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| Format: | Article |
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Wiley
2022-01-01
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| Series: | Advances in Pharmacological and Pharmaceutical Sciences |
| Online Access: | http://dx.doi.org/10.1155/2022/2156629 |
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| author | Fatemeh Akbari Mohammad Azadbakht Ali Bagheri Lale Vahedi |
| author_facet | Fatemeh Akbari Mohammad Azadbakht Ali Bagheri Lale Vahedi |
| author_sort | Fatemeh Akbari |
| collection | DOAJ |
| description | Background and Objectives. A wound is one of the high-prevalence disorders that affect people’s lives physically, mentally, and financially. This study examined the Astragalus microcephalus Willd. wound healing process in in vivo and in vitro conditions by focusing on the phytoestrogen activity of this extract. Methods. The methanolic root extract was prepared by maceration, and flavonoids were evaluated by LC/MS. In silico examination was performed based on the LC/MS results, and the binding affinity of these compounds to estrogen receptors (ERs) α and β was evaluated. Wound healing evaluation in both in vitro (NHDF cell line, by 500 μg/ml concentration of the extract, 24 h) and in vivo (Wistar rat, topical daily treated with 1.5% of the extract ointment, 21 days) conditions in comparison to control groups was conducted. Rats’ control groups included silver sulfadiazine, Vaseline, and the nontreated groups. Results. Eleven flavonoids were detected using LC/MS. The in silico study showed that formononetin, kaempferol-based structures, quercetin-3-O-neohesperidoside, and calycosin-7-O-beta-D-glucoside had a high affinity (<−6.3) to ERs α and β. Wound closing measurement showed significant improvement in the group treated with the extract in both in vitro and in vivo assays compared to the control groups. Histopathological results confirmed these findings; inflammation factors decreased, and fibroblast proliferation, fibrosis, and epithelization increased, especially in the extract group. Conclusion. This study shows that Astragalus microcephalus has wound healing activity in vitro and in vivo with low toxicity due to the presence of flavonoids, especially isoflavonoids, which show a high affinity to bind to ERs α and β in the skin tissue. |
| format | Article |
| id | doaj-art-bc5574d4298941c4a2850e93f25812fe |
| institution | Kabale University |
| issn | 2633-4690 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological and Pharmaceutical Sciences |
| spelling | doaj-art-bc5574d4298941c4a2850e93f25812fe2025-02-03T05:57:31ZengWileyAdvances in Pharmacological and Pharmaceutical Sciences2633-46902022-01-01202210.1155/2022/2156629In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.Fatemeh Akbari0Mohammad Azadbakht1Ali Bagheri2Lale Vahedi3Department of PharmacognosyDepartment of PharmacognosyDepartment of Plant and Animal BiologyDepartment of PathologyBackground and Objectives. A wound is one of the high-prevalence disorders that affect people’s lives physically, mentally, and financially. This study examined the Astragalus microcephalus Willd. wound healing process in in vivo and in vitro conditions by focusing on the phytoestrogen activity of this extract. Methods. The methanolic root extract was prepared by maceration, and flavonoids were evaluated by LC/MS. In silico examination was performed based on the LC/MS results, and the binding affinity of these compounds to estrogen receptors (ERs) α and β was evaluated. Wound healing evaluation in both in vitro (NHDF cell line, by 500 μg/ml concentration of the extract, 24 h) and in vivo (Wistar rat, topical daily treated with 1.5% of the extract ointment, 21 days) conditions in comparison to control groups was conducted. Rats’ control groups included silver sulfadiazine, Vaseline, and the nontreated groups. Results. Eleven flavonoids were detected using LC/MS. The in silico study showed that formononetin, kaempferol-based structures, quercetin-3-O-neohesperidoside, and calycosin-7-O-beta-D-glucoside had a high affinity (<−6.3) to ERs α and β. Wound closing measurement showed significant improvement in the group treated with the extract in both in vitro and in vivo assays compared to the control groups. Histopathological results confirmed these findings; inflammation factors decreased, and fibroblast proliferation, fibrosis, and epithelization increased, especially in the extract group. Conclusion. This study shows that Astragalus microcephalus has wound healing activity in vitro and in vivo with low toxicity due to the presence of flavonoids, especially isoflavonoids, which show a high affinity to bind to ERs α and β in the skin tissue.http://dx.doi.org/10.1155/2022/2156629 |
| spellingShingle | Fatemeh Akbari Mohammad Azadbakht Ali Bagheri Lale Vahedi In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd. Advances in Pharmacological and Pharmaceutical Sciences |
| title | In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd. |
| title_full | In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd. |
| title_fullStr | In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd. |
| title_full_unstemmed | In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd. |
| title_short | In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd. |
| title_sort | in silico in vitro and in vivo wound healing activity of astragalus microcephalus willd |
| url | http://dx.doi.org/10.1155/2022/2156629 |
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