Systematic comparison and base-editing-mediated directed protein evolution and functional screening yield superior auxin-inducible degron technology

Abstract Biological mechanisms are inherently dynamic, requiring precise and rapid manipulations for effective characterization. Traditional genetic manipulations operate on long timescales, making them unsuitable for studying dynamic processes or characterizing essential genes, where chronic deplet...

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Bibliographic Details
Main Authors: De Xing, Tao Bai, Ozlem Neyisci, Seyedeh Zahra Paylakhi, Alexander J. Duval, Yasemin Tekin, Mazhar Adli
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-61848-1
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