Do we need a standardized 16S rRNA gene amplicon sequencing analysis protocol for poultry microbiota research?

Do we need a standardized 16S rRNA gene amplicon sequencing analysis protocol for poultry microbiota research?

Bacteria are the major component of poultry gastrointestinal tract (GIT) microbiota and play an important role in host health, nutrition, physiology regulation, intestinal development, and growth. Bacterial community profiling based on the 16S ribosomal RNA (rRNA) gene amplicon sequencing approach h...

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Bibliographic Details
Main Authors: Joshua M. Lyte, Mitiku M. Seyoum, Diana Ayala, Jannigje G. Kers, Valentina Caputi, Timothy Johnson, Li Zhang, Joshua Rehberger, Guolong Zhang, Sami Dridi, Brett Hale, Jean E. De Oliveira, Daniel Grum, Alexandra H. Smith, Michael Kogut, Steven C. Ricke, Anne Ballou, Bill Potter, Monika Proszkowiec-Weglarz
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Poultry Science
Subjects:
16S rRNA gene sequencing
Standardized protocol
Next-generation sequencing
Poultry
Microbiota
Online Access:http://www.sciencedirect.com/science/article/pii/S0032579125004845
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