High-Throughput Method for Isolating Plasmid DNA with Reduced Lipopolysaccharide Content
Isolating plasmid DNA from bacteria is a fundamental step in molecular biology. It is often accomplished by an alkaline lysis of bacteria and the subsequent adsorption of nucleic acids to silica oxide in the presence of chaotropic substances. Here we show that the addition of such chaotropic reagent...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2000-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/00281st06 |
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