Thermus thermophilus CRISPR Cas6 Heterologous Expression and Purification
The CRISPR-Cas system of Thermus thermophilus has emerged as a potent biotechnological tool, particularly its Cas6 endonuclease, which plays a crucial role in CRISPR RNA (crRNA) maturation. This protocol details a robust and reproducible method for the high-level expression and purification of recom...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2025-07-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5382&type=0 |
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