Enterobacter hormaechei: an endophytic bacterium found in Avocado Peel (Persea americana Mill.) with antioxidant properties
Avocado peels (AVP) are renowned for their potent antioxidant properties, making them highly effective in preventing oxidation and free radical formation. Endophytes, microorganisms residing within plant tissues, have demonstrated the ability to produce novel compounds with remarkable biological ac...
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| Format: | Article |
| Language: | English |
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Universitas Ahmad Dahlan
2024-11-01
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| Series: | Pharmaciana |
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| Online Access: | https://journal.uad.ac.id/index.php/PHARMACIANA/article/view/28432 |
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| author | Rustini Rustini Khalila Rahmi Rahmi Purnawan Pontana Putra Regina Andayani Andayani Khiky Dwinatrana |
| author_facet | Rustini Rustini Khalila Rahmi Rahmi Purnawan Pontana Putra Regina Andayani Andayani Khiky Dwinatrana |
| author_sort | Rustini Rustini |
| collection | DOAJ |
| description |
Avocado peels (AVP) are renowned for their potent antioxidant properties, making them highly effective in preventing oxidation and free radical formation. Endophytes, microorganisms residing within plant tissues, have demonstrated the ability to produce novel compounds with remarkable biological activities. These bioactive compounds are sometimes even more potent than those found in their host plants. This study explores the potential of endophytes from avocado peels as rich sources of antioxidant compounds. AVP samples are collected, surface-sterilized, and segmented before being cultured in growth media. The bacteria are then isolated, purified, and subjected to ethyl acetate extraction to evaluate their antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) microassay. Samples demonstrating favorable antioxidant properties undergo molecular identification through 16S rRNA gene sequencing. Four bacterial strains are successfully isolated, with only the APK4 strain exhibiting significant antioxidant activity with an IC50 value of 302.3 μg/mL. Molecular analysis and phylogenetic tree construction reveal that APK4 is closely related to the Enterobacter hormaechei species, with a percent identity value of 99.93%. These findings highlight the potential of active metabolites from endophytic bacteria in AVP extracts as promising lead compounds for the development of novel drugs, nutraceuticals, and cosmetic ingredients.
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| format | Article |
| id | doaj-art-ba6581fa56154b3dbc0cf326a95fce4e |
| institution | Kabale University |
| issn | 2088-4559 2477-0256 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Universitas Ahmad Dahlan |
| record_format | Article |
| series | Pharmaciana |
| spelling | doaj-art-ba6581fa56154b3dbc0cf326a95fce4e2025-01-12T00:37:06ZengUniversitas Ahmad DahlanPharmaciana2088-45592477-02562024-11-0114310.12928/pharmaciana.v14i3.2843220508Enterobacter hormaechei: an endophytic bacterium found in Avocado Peel (Persea americana Mill.) with antioxidant properties Rustini Rustini0Khalila Rahmi Rahmi1Purnawan Pontana Putra2Regina Andayani Andayani3Khiky Dwinatrana4Faculty of Pharmacy, Universitas AndalasFaculty of Pharmacy, Universitas AndalasFaculty of Pharmacy, Universitas AndalasFaculty of Pharmacy, Universitas AndalasDepartment of Pharmacy, University of Dharma Andalas Avocado peels (AVP) are renowned for their potent antioxidant properties, making them highly effective in preventing oxidation and free radical formation. Endophytes, microorganisms residing within plant tissues, have demonstrated the ability to produce novel compounds with remarkable biological activities. These bioactive compounds are sometimes even more potent than those found in their host plants. This study explores the potential of endophytes from avocado peels as rich sources of antioxidant compounds. AVP samples are collected, surface-sterilized, and segmented before being cultured in growth media. The bacteria are then isolated, purified, and subjected to ethyl acetate extraction to evaluate their antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) microassay. Samples demonstrating favorable antioxidant properties undergo molecular identification through 16S rRNA gene sequencing. Four bacterial strains are successfully isolated, with only the APK4 strain exhibiting significant antioxidant activity with an IC50 value of 302.3 μg/mL. Molecular analysis and phylogenetic tree construction reveal that APK4 is closely related to the Enterobacter hormaechei species, with a percent identity value of 99.93%. These findings highlight the potential of active metabolites from endophytic bacteria in AVP extracts as promising lead compounds for the development of novel drugs, nutraceuticals, and cosmetic ingredients. https://journal.uad.ac.id/index.php/PHARMACIANA/article/view/28432Avocado peelEndophytic bacteriaEnterobacter hormacheiAntioxidant activity |
| spellingShingle | Rustini Rustini Khalila Rahmi Rahmi Purnawan Pontana Putra Regina Andayani Andayani Khiky Dwinatrana Enterobacter hormaechei: an endophytic bacterium found in Avocado Peel (Persea americana Mill.) with antioxidant properties Pharmaciana Avocado peel Endophytic bacteria Enterobacter hormachei Antioxidant activity |
| title | Enterobacter hormaechei: an endophytic bacterium found in Avocado Peel (Persea americana Mill.) with antioxidant properties |
| title_full | Enterobacter hormaechei: an endophytic bacterium found in Avocado Peel (Persea americana Mill.) with antioxidant properties |
| title_fullStr | Enterobacter hormaechei: an endophytic bacterium found in Avocado Peel (Persea americana Mill.) with antioxidant properties |
| title_full_unstemmed | Enterobacter hormaechei: an endophytic bacterium found in Avocado Peel (Persea americana Mill.) with antioxidant properties |
| title_short | Enterobacter hormaechei: an endophytic bacterium found in Avocado Peel (Persea americana Mill.) with antioxidant properties |
| title_sort | enterobacter hormaechei an endophytic bacterium found in avocado peel persea americana mill with antioxidant properties |
| topic | Avocado peel Endophytic bacteria Enterobacter hormachei Antioxidant activity |
| url | https://journal.uad.ac.id/index.php/PHARMACIANA/article/view/28432 |
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