Protocol for differential analysis of pseudouridine modifications using nanopore DRS and unmodified transcriptome control

Protocol for differential analysis of pseudouridine modifications using nanopore DRS and unmodified transcriptome control

Summary: In this protocol, we detect pseudouridine using nanopore direct RNA sequencing (DRS 002/004) and analyze dynamic changes to mRNA modifications in response to perturbation. We cover RNA extraction, poly(A) selection, DRS, unmodified transcriptome (in vitro transcribed [IVT]) library preparat...

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Bibliographic Details
Main Authors: Oleksandra Fanari, Michele Meseonznik, Dylan Bloch, Sara H. Rouhanifard
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Bioinformatics
Sequence analysis
Genetics
Genomics
RNA-seq
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003545
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http://www.sciencedirect.com/science/article/pii/S2666166725003545

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