Sensitive detection of gene expression in mycobacteria under replicating and non-replicating conditions using optimized far-red reporters.
Fluorescent reporter proteins have proven useful for imaging techniques in many organisms. We constructed optimized expression systems for several fluorescent proteins from the far-red region of the spectrum and analyzed their utility in several mycobacterial species. Plasmids expressing variants of...
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| Main Authors: | Paul Carroll, Lise J Schreuder, Julian Muwanguzi-Karugaba, Siouxsie Wiles, Brian D Robertson, Jorge Ripoll, Theresa H Ward, Gregory J Bancroft, Ulrich E Schaible, Tanya Parish |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-03-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0009823&type=printable |
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