Sensitive detection of gene expression in mycobacteria under replicating and non-replicating conditions using optimized far-red reporters.

Sensitive detection of gene expression in mycobacteria under replicating and non-replicating conditions using optimized far-red reporters.

Fluorescent reporter proteins have proven useful for imaging techniques in many organisms. We constructed optimized expression systems for several fluorescent proteins from the far-red region of the spectrum and analyzed their utility in several mycobacterial species. Plasmids expressing variants of...

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Bibliographic Details
Main Authors: Paul Carroll, Lise J Schreuder, Julian Muwanguzi-Karugaba, Siouxsie Wiles, Brian D Robertson, Jorge Ripoll, Theresa H Ward, Gregory J Bancroft, Ulrich E Schaible, Tanya Parish
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-03-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0009823&type=printable
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https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0009823&type=printable

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