Generation of DNA nanocircles containing mismatched bases

The DNA mismatch repair (MMR) system recognizes and repairs errors that escaped the proofreading function of DNA polymerases. To study molecular details of the MMR mechanism, in vitro biochemical assays require specific DNA substrates carrying mismatches and strand discrimination signals. Current ap...

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Bibliographic Details
Main Authors: Yu Xiao, Caroline Jung, Andreas D. Marx, Ines Winkler, Claire Wyman, Joyce H.G. Lebbink, Peter Friedhoff, Michele Cristovao
Format: Article
Language:English
Published: Taylor & Francis Group 2011-10-01
Series:BioTechniques
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Online Access:https://www.future-science.com/doi/10.2144/000113749
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