PCR Mutagenesis-Based Method for Generation of Positive Controls for SSCP Analysis

We have developed a primer-mediated PCR mutagenesis-based method for the generation of positive controls to test the sensitivity of single-strand conformation polymorphism (SSCP) or any other PCRbased mutation screening method. This technique is based on the incorporation of a third longer primer, c...

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Bibliographic Details
Main Authors: A. Ruiz, G. Antiñolo, S. Borrego, B. Sánchez, J. Sánchez
Format: Article
Language:English
Published: Taylor & Francis Group 1997-10-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/97234st04
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