Effect of EGCG–Methacrylate-Functionalized Resin Infiltrant on White Spot Lesions: An In Vitro Study
This study evaluated the color change (ΔE<sub>00</sub>) and penetration depth (PD) of white spot lesions (WSLs) infiltrated with the resin infiltrant (Icon<sup>®</sup>) functionalized with methacrylate epigallocatechin-3-gallate (EGCG). To introduce polymerizable double bonds...
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| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/16/1/6 |
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| author | Karin Landmayer Bruna de Oliveira Iatarola Talita Portela Pereira Raquel Shimizu Mori Alyssa Teixeira Obeid Mariele Vertuan Daniela Alvim Chrisostomo Ana Carolina Magalhães Lulwah Alreshaid Paulo Henrique dos Santos Anuradha Prakki Luciana Fávaro Francisconi-dos-Rios |
| author_facet | Karin Landmayer Bruna de Oliveira Iatarola Talita Portela Pereira Raquel Shimizu Mori Alyssa Teixeira Obeid Mariele Vertuan Daniela Alvim Chrisostomo Ana Carolina Magalhães Lulwah Alreshaid Paulo Henrique dos Santos Anuradha Prakki Luciana Fávaro Francisconi-dos-Rios |
| author_sort | Karin Landmayer |
| collection | DOAJ |
| description | This study evaluated the color change (ΔE<sub>00</sub>) and penetration depth (PD) of white spot lesions (WSLs) infiltrated with the resin infiltrant (Icon<sup>®</sup>) functionalized with methacrylate epigallocatechin-3-gallate (EGCG). To introduce polymerizable double bonds, EGCG was reacted with methacryloyl chloride (EM). Subsequently, the Icon resin infiltrant (I) was loaded with neat EGCG (IE) or EGCG–methacrylate (IEM) at 2 wt% each. WSLs were created on bovine enamel blocks and treated with I, IE, or IEM. Sound and untreated enamel surfaces were used as controls (C). Infiltrant PD (%) was determined by Confocal Laser Scanning Microscopy (CLSM, n = 12) analysis. For color change (ΔE<sub>00</sub>) determination (n = 14), ΔL, Δa, and Δb, half of each sample was kept sound as a reference area. The color was determined with a spectrophotometer. Data were statistically evaluated (<i>p</i> = 0.05). Surface morphology was obtained as a qualitative response variable using 3D CLSM. PD (%) did not differ statistically for I, IE, and IEM (<i>p</i> = 0.780). Groups I and IEM showed similar performance on color change (ΔE<sub>00</sub>) compared to the control group, while IE exhibited intermediate results, with no significant difference observed between the untreated, I, and IEM groups (<i>p</i> < 0.001). IEM promoted the masking of the WSL color without interfering with the PD. |
| format | Article |
| id | doaj-art-b8de380f4e744f5faadd732b3eaa5a84 |
| institution | Kabale University |
| issn | 2079-4983 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj-art-b8de380f4e744f5faadd732b3eaa5a842025-01-24T13:36:05ZengMDPI AGJournal of Functional Biomaterials2079-49832024-12-01161610.3390/jfb16010006Effect of EGCG–Methacrylate-Functionalized Resin Infiltrant on White Spot Lesions: An In Vitro StudyKarin Landmayer0Bruna de Oliveira Iatarola1Talita Portela Pereira2Raquel Shimizu Mori3Alyssa Teixeira Obeid4Mariele Vertuan5Daniela Alvim Chrisostomo6Ana Carolina Magalhães7Lulwah Alreshaid8Paulo Henrique dos Santos9Anuradha Prakki10Luciana Fávaro Francisconi-dos-Rios11Restorative Discipline, Dental Research Institute, Faculty of Dentistry, University of Toronto, 101 Elm Street, Toronto, ON M5G 1G6, CanadaDepartment of Operative Dentistry, School of Dentistry, University of São Paulo, Avenida Professor Lineu Prestes, 2227, São Paulo 05508-000, SP, BrazilDepartment of Operative Dentistry, School of Dentistry, University of São Paulo, Avenida Professor Lineu Prestes, 2227, São Paulo 05508-000, SP, BrazilDepartment of Operative Dentistry, School of Dentistry, University of São Paulo, Avenida Professor Lineu Prestes, 2227, São Paulo 05508-000, SP, BrazilDepartment of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla, 9-75, Bauru 17012-901, SP, BrazilDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla, 9-75, Bauru 17012-901, SP, BrazilRestorative Discipline, Dental Research Institute, Faculty of Dentistry, University of Toronto, 101 Elm Street, Toronto, ON M5G 1G6, CanadaDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla, 9-75, Bauru 17012-901, SP, BrazilDepartment of Restorative and Prosthetic Dental Sciences, College of Dentistry, King Saud bin Abdulaziz University for Health Sciences, P.O. Box 3660, Riyadh 11481 K.S.A, Saudi ArabiaRestorative Discipline, Dental Research Institute, Faculty of Dentistry, University of Toronto, 101 Elm Street, Toronto, ON M5G 1G6, CanadaRestorative Discipline, Dental Research Institute, Faculty of Dentistry, University of Toronto, 101 Elm Street, Toronto, ON M5G 1G6, CanadaDepartment of Operative Dentistry, School of Dentistry, University of São Paulo, Avenida Professor Lineu Prestes, 2227, São Paulo 05508-000, SP, BrazilThis study evaluated the color change (ΔE<sub>00</sub>) and penetration depth (PD) of white spot lesions (WSLs) infiltrated with the resin infiltrant (Icon<sup>®</sup>) functionalized with methacrylate epigallocatechin-3-gallate (EGCG). To introduce polymerizable double bonds, EGCG was reacted with methacryloyl chloride (EM). Subsequently, the Icon resin infiltrant (I) was loaded with neat EGCG (IE) or EGCG–methacrylate (IEM) at 2 wt% each. WSLs were created on bovine enamel blocks and treated with I, IE, or IEM. Sound and untreated enamel surfaces were used as controls (C). Infiltrant PD (%) was determined by Confocal Laser Scanning Microscopy (CLSM, n = 12) analysis. For color change (ΔE<sub>00</sub>) determination (n = 14), ΔL, Δa, and Δb, half of each sample was kept sound as a reference area. The color was determined with a spectrophotometer. Data were statistically evaluated (<i>p</i> = 0.05). Surface morphology was obtained as a qualitative response variable using 3D CLSM. PD (%) did not differ statistically for I, IE, and IEM (<i>p</i> = 0.780). Groups I and IEM showed similar performance on color change (ΔE<sub>00</sub>) compared to the control group, while IE exhibited intermediate results, with no significant difference observed between the untreated, I, and IEM groups (<i>p</i> < 0.001). IEM promoted the masking of the WSL color without interfering with the PD.https://www.mdpi.com/2079-4983/16/1/6antimicrobial agentsconfocal laser scanning microscopydental white spotscatechinicon infiltrantultraviolet spectrophotometry |
| spellingShingle | Karin Landmayer Bruna de Oliveira Iatarola Talita Portela Pereira Raquel Shimizu Mori Alyssa Teixeira Obeid Mariele Vertuan Daniela Alvim Chrisostomo Ana Carolina Magalhães Lulwah Alreshaid Paulo Henrique dos Santos Anuradha Prakki Luciana Fávaro Francisconi-dos-Rios Effect of EGCG–Methacrylate-Functionalized Resin Infiltrant on White Spot Lesions: An In Vitro Study Journal of Functional Biomaterials antimicrobial agents confocal laser scanning microscopy dental white spots catechin icon infiltrant ultraviolet spectrophotometry |
| title | Effect of EGCG–Methacrylate-Functionalized Resin Infiltrant on White Spot Lesions: An In Vitro Study |
| title_full | Effect of EGCG–Methacrylate-Functionalized Resin Infiltrant on White Spot Lesions: An In Vitro Study |
| title_fullStr | Effect of EGCG–Methacrylate-Functionalized Resin Infiltrant on White Spot Lesions: An In Vitro Study |
| title_full_unstemmed | Effect of EGCG–Methacrylate-Functionalized Resin Infiltrant on White Spot Lesions: An In Vitro Study |
| title_short | Effect of EGCG–Methacrylate-Functionalized Resin Infiltrant on White Spot Lesions: An In Vitro Study |
| title_sort | effect of egcg methacrylate functionalized resin infiltrant on white spot lesions an in vitro study |
| topic | antimicrobial agents confocal laser scanning microscopy dental white spots catechin icon infiltrant ultraviolet spectrophotometry |
| url | https://www.mdpi.com/2079-4983/16/1/6 |
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