Fusion Proteins Could Generate False Positives in Peptide Phage Display
Fusion proteins are frequently used in the functional characterization of newly discovered proteins and to identify interacting partners. In our study of hPTP1E, a cytosolic protein tyrosine phosphatase, we used glutathione S-transferase (GST)-fusion protein of the second PDZ domain to identify inte...
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| Main Authors: | Kishore K. Murthy, Irena Ekiel, Shi-Hsiang Shen, Denis Banville |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1999-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/99261rr03 |
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