Fusion Proteins Could Generate False Positives in Peptide Phage Display

Fusion proteins are frequently used in the functional characterization of newly discovered proteins and to identify interacting partners. In our study of hPTP1E, a cytosolic protein tyrosine phosphatase, we used glutathione S-transferase (GST)-fusion protein of the second PDZ domain to identify inte...

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Bibliographic Details
Main Authors: Kishore K. Murthy, Irena Ekiel, Shi-Hsiang Shen, Denis Banville
Format: Article
Language:English
Published: Taylor & Francis Group 1999-01-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/99261rr03
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