Fluorescence Lifetime Multiplexing with Fluorogen-Activating FAST Protein Variants and Red-Shifted Arylidene–Imidazolone Derivative as Fluorogen

Fluorescence Lifetime Multiplexing with Fluorogen-Activating FAST Protein Variants and Red-Shifted Arylidene–Imidazolone Derivative as Fluorogen

Fluorescence-lifetime imaging microscopy (FLIM) is a powerful technique for highly multiplexed imaging in live cells. In this work, we present a genetically encoded FLIM multiplexing platform based on a combination of fluorogen-activating protein FAST and red-shifted fluorogen <b>N871b</b&g...

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Bibliographic Details
Main Authors: Aidar R. Gilvanov, Ivan N. Myasnyanko, Sergey A. Goncharuk, Marina V. Goncharuk, Vadim S. Kublitski, Daria V. Bodunova, Svetlana V. Sidorenko, Eugene G. Maksimov, Mikhail S. Baranov, Yulia A. Bogdanova
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Biosensors
Subjects:
arylidene–imidazolone
FLIM
fluorogen-activating protein
Online Access:https://www.mdpi.com/2079-6374/15/5/274
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