Hepatocyte Differentiation from Human ES Cells using the Simple Embryoid Body Formation Method and the Staged-Additional Cocktail

Hepatocyte Differentiation from Human ES Cells using the Simple Embryoid Body Formation Method and the Staged-Additional Cocktail

To induce hepatocytes from human embryonic stem (hES) cells easily and effectively, a simple suspension culture method that separates ES colonies with a scraper and transfers them into newly developed, nonadherent MPC (2-methacryloyloxyethyl phosphorylcholine) plates, and the staged-additional cockt...

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Main Authors: Katsunori Sasaki, Hinako Ichikawa, Shunsuke Takei, Hee Sung No, Daihachiro Tomotsune, Yoshiya Kano, Tadayuki Yokoyama, Sakiko Sirasawa, Akimi Mogi, Susumu Yoshie, Shujiro Sasaki, Satoshi Yamada, Ken Matsumoto, Masahiro Mizuguchi, Fengming Yue, Yoshiki Tanaka
Format: Article
Language:English
Published: Wiley 2009-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/tsw.2009.97
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http://dx.doi.org/10.1100/tsw.2009.97

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