Highly Sensitive Immunosensing of Carcinoembryonic Antigen Based on Gold Nanoparticles Dotted PB@PANI Core-Shell Nanocubes as a Signal Probe
Herein, a method was developed for the sensitive monitoring of carcinoembryonic antigen (CEA) by gold nanoparticles dotted prussian blue@polyaniline core-shell nanocubes (Au NPs/PB@PANI). First, a facile low-temperature method was used to prepare the uniform PB@PANI core-shell nanocubes with the ass...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2023/7009624 |
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| author | Dexiang Feng Lingzhi Chen Ke Zhang Shuangshuang Zhu Meichen Ying Peng Jiang Menglan Fu Yan Wei Lihua Li |
| author_facet | Dexiang Feng Lingzhi Chen Ke Zhang Shuangshuang Zhu Meichen Ying Peng Jiang Menglan Fu Yan Wei Lihua Li |
| author_sort | Dexiang Feng |
| collection | DOAJ |
| description | Herein, a method was developed for the sensitive monitoring of carcinoembryonic antigen (CEA) by gold nanoparticles dotted prussian blue@polyaniline core-shell nanocubes (Au NPs/PB@PANI). First, a facile low-temperature method was used to prepare the uniform PB@PANI core-shell nanocubes with the assistance of PVP, where PB acted as the electron transfer mediator to provide electrochemical signals, and the PANI with excellent conductivity and desirable chemical stability not only played the role of a protective layer to prevent etching of PB in basic media but also effectively improved electron transfer. Importantly, to further enhance the electrical conductivity and biocompatibility of PB@PANI and to further enhance the electrochemical signal and capture a large amount of Ab2, Au NPs were doped on the surface of PB@PANI to form Au NPs/PB@PANI nanocomposites. Subsequently, benefiting from the advantages of core-shell structure nanoprobes and gold-platinum bimetallic nanoflower (AuPt NF), a sandwich-type electrochemical immunosensor for CEA detection was constructed, which provided a wide linear detection range from 1.0 pg·mL−1 to 100.0 ng·mL−1 and a low detection limit of 0.35 pg·mL−1 via DPV (at 3σ). Moreover, it displayed a satisfactory result when the core-shell structure nanoprobe-based immunosensor was applied to determine CEA in real human serum samples. |
| format | Article |
| id | doaj-art-b7824055fffc41e395b1e15ee2244d11 |
| institution | Kabale University |
| issn | 2090-8873 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-b7824055fffc41e395b1e15ee2244d112025-02-03T06:42:53ZengWileyJournal of Analytical Methods in Chemistry2090-88732023-01-01202310.1155/2023/7009624Highly Sensitive Immunosensing of Carcinoembryonic Antigen Based on Gold Nanoparticles Dotted PB@PANI Core-Shell Nanocubes as a Signal ProbeDexiang Feng0Lingzhi Chen1Ke Zhang2Shuangshuang Zhu3Meichen Ying4Peng Jiang5Menglan Fu6Yan Wei7Lihua Li8Department of ChemistryInstitute of Synthesis and Application of Medical MaterialsDepartment of ChemistryDepartment of ChemistryInstitute of Synthesis and Application of Medical MaterialsInstitute of Synthesis and Application of Medical MaterialsInstitute of Synthesis and Application of Medical MaterialsDepartment of ChemistryInstitute of Synthesis and Application of Medical MaterialsHerein, a method was developed for the sensitive monitoring of carcinoembryonic antigen (CEA) by gold nanoparticles dotted prussian blue@polyaniline core-shell nanocubes (Au NPs/PB@PANI). First, a facile low-temperature method was used to prepare the uniform PB@PANI core-shell nanocubes with the assistance of PVP, where PB acted as the electron transfer mediator to provide electrochemical signals, and the PANI with excellent conductivity and desirable chemical stability not only played the role of a protective layer to prevent etching of PB in basic media but also effectively improved electron transfer. Importantly, to further enhance the electrical conductivity and biocompatibility of PB@PANI and to further enhance the electrochemical signal and capture a large amount of Ab2, Au NPs were doped on the surface of PB@PANI to form Au NPs/PB@PANI nanocomposites. Subsequently, benefiting from the advantages of core-shell structure nanoprobes and gold-platinum bimetallic nanoflower (AuPt NF), a sandwich-type electrochemical immunosensor for CEA detection was constructed, which provided a wide linear detection range from 1.0 pg·mL−1 to 100.0 ng·mL−1 and a low detection limit of 0.35 pg·mL−1 via DPV (at 3σ). Moreover, it displayed a satisfactory result when the core-shell structure nanoprobe-based immunosensor was applied to determine CEA in real human serum samples.http://dx.doi.org/10.1155/2023/7009624 |
| spellingShingle | Dexiang Feng Lingzhi Chen Ke Zhang Shuangshuang Zhu Meichen Ying Peng Jiang Menglan Fu Yan Wei Lihua Li Highly Sensitive Immunosensing of Carcinoembryonic Antigen Based on Gold Nanoparticles Dotted PB@PANI Core-Shell Nanocubes as a Signal Probe Journal of Analytical Methods in Chemistry |
| title | Highly Sensitive Immunosensing of Carcinoembryonic Antigen Based on Gold Nanoparticles Dotted PB@PANI Core-Shell Nanocubes as a Signal Probe |
| title_full | Highly Sensitive Immunosensing of Carcinoembryonic Antigen Based on Gold Nanoparticles Dotted PB@PANI Core-Shell Nanocubes as a Signal Probe |
| title_fullStr | Highly Sensitive Immunosensing of Carcinoembryonic Antigen Based on Gold Nanoparticles Dotted PB@PANI Core-Shell Nanocubes as a Signal Probe |
| title_full_unstemmed | Highly Sensitive Immunosensing of Carcinoembryonic Antigen Based on Gold Nanoparticles Dotted PB@PANI Core-Shell Nanocubes as a Signal Probe |
| title_short | Highly Sensitive Immunosensing of Carcinoembryonic Antigen Based on Gold Nanoparticles Dotted PB@PANI Core-Shell Nanocubes as a Signal Probe |
| title_sort | highly sensitive immunosensing of carcinoembryonic antigen based on gold nanoparticles dotted pb pani core shell nanocubes as a signal probe |
| url | http://dx.doi.org/10.1155/2023/7009624 |
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