Optimized Protocol for High-Quality RNA Extraction from Grape Berry Skins Using Sorbitol Pre-Wash

Optimized Protocol for High-Quality RNA Extraction from Grape Berry Skins Using Sorbitol Pre-Wash

Extracting high-quality RNA from grape (<i>Vitis Vinifera</i>) berry skins is challenging due to their high levels of polysaccharides, phenolic compounds, sugars, and organic acids, which can negatively impact RNA purity and yield. Indeed, polyphenols can bind to RNA, polysaccharides may...

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Bibliographic Details
Main Authors: Annalisa Prencipe, Antonella Salerno, Margherita D’Amico, Antonio Domenico Marsico, Mario Ventura, Riccardo Velasco, Maria Francesca Cardone, Carlo Bergamini, Flavia Angela Maria Maggiolini
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Plants
Subjects:
<i>Vitis vinifera</i>
RNA
sorbitol
extraction protocol
Online Access:https://www.mdpi.com/2223-7747/14/7/988
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