Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in <i>Pichia pastoris</i>
Enzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation. Microbial heterologous expression systems ha...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-5354/12/1/68 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Enzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation. Microbial heterologous expression systems have been studied for meeting this demand. Among these systems, the <i>Pichia pastoris</i> expression system has emerged as a widely used platform for producing secreted heterologous proteins. This article provides an overview of studies involving the recombinant expression of polymer-degrading enzymes using the <i>P. pastoris</i> expression system. Research on <i>P. pastoris</i> expression of interested enzymes with depolymerising ability, including cutinase, lipase, and laccase, are highlighted in the review. The key factors influencing the heterologous expression of polymer-degrading enzymes in <i>P. pastoris</i> are discussed, shedding light on the challenges and opportunities in the development of depolymerising biocatalysts through the <i>P. pastoris</i> expression system. |
|---|---|
| ISSN: | 2306-5354 |