Most human DNA replication initiation is dispersed throughout the genome with only a minority within previously identified initiation zones

Most human DNA replication initiation is dispersed throughout the genome with only a minority within previously identified initiation zones

Abstract Background The identification of sites of DNA replication initiation in mammalian cells has been challenging. Here, we present unbiased detection of replication initiation events in human cells using BrdU incorporation and single-molecule nanopore sequencing. Results Increases in BrdU incor...

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Main Authors: Jamie T. Carrington, Rosemary H. C. Wilson, Eduardo de La Vega, Sathish Thiyagarajan, Tom Barker, Leah Catchpole, Alex Durrant, Vanda Knitlhoffer, Chris Watkins, Karim Gharbi, Conrad A. Nieduszynski
Format: Article
Language:English
Published: BMC 2025-05-01
Series:Genome Biology
Subjects:
Replication origin
Origin mapping
DNAscent
Ultra-long
nCATS
Online Access:https://doi.org/10.1186/s13059-025-03591-w
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https://doi.org/10.1186/s13059-025-03591-w

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