Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuA

Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuA

Summary: OpuA is an osmoregulatory ATP-binding cassette transporter that undergoes different conformations upon varying salt concentrations and ligand conditions. Here, we present a protocol to study unfolding and interdomain interactions of OpuA using single-molecule optical tweezers (smOT). We des...

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Bibliographic Details
Main Authors: Lyan van der Sleen, Marco van den Noort, Laura-Marie Silbermann, Bert Poolman, Katarzyna Tych
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Biophysics
Single-molecule assays
Protein biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002758
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http://www.sciencedirect.com/science/article/pii/S2666166725002758

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