3D printed lactic acid bacteria hydrogel: cell release kinetics and stability
In this study, a new type of 3D printed living biological hydrogel was developed by integrating lactic acid bacteria (LAB) into biocompatible and non-toxic polymer materials. Interestingly, the living materials loaded with LAB can be freeze-dried and reused for more than 100 times. The bio-hydrogel...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-03-01
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| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001616 |
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| author | Yifei Liu Xintao Yin Xiudong Xia Zhen Liu Lifei Chen Mingsheng Dong |
| author_facet | Yifei Liu Xintao Yin Xiudong Xia Zhen Liu Lifei Chen Mingsheng Dong |
| author_sort | Yifei Liu |
| collection | DOAJ |
| description | In this study, a new type of 3D printed living biological hydrogel was developed by integrating lactic acid bacteria (LAB) into biocompatible and non-toxic polymer materials. Interestingly, the living materials loaded with LAB can be freeze-dried and reused for more than 100 times. The bio-hydrogel can be used to co-culture different LAB and keep its fermentation performance stable in long-term use. The release kinetics model and response surface method were used to simulate and optimize the bacteria release mode in the bio-hydrogel. The results show that the release of bacteria from hydrogel is regulated by the coupling of Fickian diffusion and polymer swelling. The stability of LAB hydrogel was evaluated by reuse experiments. The images of confocal microscopy and scanning electron microscope showed that the bacteria with high cell viability were distributed in the hydrogel and intact structure of the living hydrogel was maintained after 100 times of reuse as yoghurt starter. In conclusion, the 3D printed LAB bio-hydrogel developed in this study has the advantage of reuse and sustainability, which is expected to open up a new way for the preparation of food culture starter. |
| format | Article |
| id | doaj-art-b6883863c26e48769c119b4ce01d361c |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-b6883863c26e48769c119b4ce01d361c2025-02-02T23:24:43ZengTsinghua University PressFood Science and Human Wellness2213-45302023-03-011224774873D printed lactic acid bacteria hydrogel: cell release kinetics and stabilityYifei Liu0Xintao Yin1Xiudong Xia2Zhen Liu3Lifei Chen4Mingsheng Dong5College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute of Agricultural Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Corresponding author at: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.In this study, a new type of 3D printed living biological hydrogel was developed by integrating lactic acid bacteria (LAB) into biocompatible and non-toxic polymer materials. Interestingly, the living materials loaded with LAB can be freeze-dried and reused for more than 100 times. The bio-hydrogel can be used to co-culture different LAB and keep its fermentation performance stable in long-term use. The release kinetics model and response surface method were used to simulate and optimize the bacteria release mode in the bio-hydrogel. The results show that the release of bacteria from hydrogel is regulated by the coupling of Fickian diffusion and polymer swelling. The stability of LAB hydrogel was evaluated by reuse experiments. The images of confocal microscopy and scanning electron microscope showed that the bacteria with high cell viability were distributed in the hydrogel and intact structure of the living hydrogel was maintained after 100 times of reuse as yoghurt starter. In conclusion, the 3D printed LAB bio-hydrogel developed in this study has the advantage of reuse and sustainability, which is expected to open up a new way for the preparation of food culture starter.http://www.sciencedirect.com/science/article/pii/S22134530220016163D printingBioinkLiving hydrogelLactic acid bacteriaCulture starter |
| spellingShingle | Yifei Liu Xintao Yin Xiudong Xia Zhen Liu Lifei Chen Mingsheng Dong 3D printed lactic acid bacteria hydrogel: cell release kinetics and stability Food Science and Human Wellness 3D printing Bioink Living hydrogel Lactic acid bacteria Culture starter |
| title | 3D printed lactic acid bacteria hydrogel: cell release kinetics and stability |
| title_full | 3D printed lactic acid bacteria hydrogel: cell release kinetics and stability |
| title_fullStr | 3D printed lactic acid bacteria hydrogel: cell release kinetics and stability |
| title_full_unstemmed | 3D printed lactic acid bacteria hydrogel: cell release kinetics and stability |
| title_short | 3D printed lactic acid bacteria hydrogel: cell release kinetics and stability |
| title_sort | 3d printed lactic acid bacteria hydrogel cell release kinetics and stability |
| topic | 3D printing Bioink Living hydrogel Lactic acid bacteria Culture starter |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001616 |
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