Phase separation of TTBK2 and CEP164 is necessary for ciliogenesis

Phase separation of TTBK2 and CEP164 is necessary for ciliogenesis

Summary: The primary cilium plays a crucial function in sensing and transmitting extracellular signals into cells. The initiation of ciliogenesis hinges on the recruitment of Tau tubulin kinase 2 (TTBK2) to the distal appendages (DAs) of centrioles through CEP164. However, the detailed mechanism und...

Full description

Saved in:
Bibliographic Details
Main Authors: Po-Chun Chou, Yu-Hao Lin, I-Hsuan Lin, Tzu-Ying Lin, Yun-Chia Huang, Shi-Rong Hong, Ting-Jui Ben Chang, Yin Fang, Yizhi Lin, T. Tony Yang, Yu-Chun Lin, Jie-rong Huang, Won-Jing Wang
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Cell Reports
Subjects:
CP: Cell biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124725005819
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary: The primary cilium plays a crucial function in sensing and transmitting extracellular signals into cells. The initiation of ciliogenesis hinges on the recruitment of Tau tubulin kinase 2 (TTBK2) to the distal appendages (DAs) of centrioles through CEP164. However, the detailed mechanism underlying the CEP164/TTBK2 interaction at DAs during ciliogenesis remains incompletely understood. In this study, we unveil that CEP164 features a long intrinsically disordered region and forms dynamic condensates with TTBK2 through phase separation. Our investigation demonstrates that CEP164 undergoes phase separation with TTBK2 through multivalent electrostatic interactions. These interactions facilitate the efficient recruitment of TTBK2 to DAs, thereby kickstarting the process of cilia formation. Therefore, our findings provide insights into the molecular regulation of CEP164/TTBK2 interactions at DAs and highlight the pivotal role of phase separation in promoting cilia formation.
ISSN:2211-1247

Similar Items