Semisynthesis of Ribonuclease A using Intein-Mediated Protein Ligation

The introduction of non-natural amino acid residues or modules into proteins provides a new means to explore the basis for conformational stability, folding/unfolding behavior, or biological function. We exploited intein-mediated protein ligation to produce a semisynthetic ribonuclease A. Of the 124...

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Bibliographic Details
Main Authors: Ulrich Arnold, Matthew P. Hinderaker, Ronald T. Raines
Format: Article
Language:English
Published: Wiley 2002-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/tsw.2002.855
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