Evaluation of PAMAM Dendrimer-Stabilized Gold Nanoparticles: Two-Stage Procedure Synthesis and Toxicity Assessment in MCF-7 Breast Cancer Cells
Gold nanoparticles stabilized with polyamidoamine dendrimers are one of the potential candidates for use as a contrast agent in computed tomography and a drug delivery agent. This work demonstrates a rapid, two-step synthesis of such complexes, which are size-stable for up to 18 months. The first st...
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MDPI AG
2025-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/30/9/2024 |
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| author | Agnieszka Maria Kołodziejczyk Magdalena Grala Łukasz Kołodziejczyk |
| author_facet | Agnieszka Maria Kołodziejczyk Magdalena Grala Łukasz Kołodziejczyk |
| author_sort | Agnieszka Maria Kołodziejczyk |
| collection | DOAJ |
| description | Gold nanoparticles stabilized with polyamidoamine dendrimers are one of the potential candidates for use as a contrast agent in computed tomography and a drug delivery agent. This work demonstrates a rapid, two-step synthesis of such complexes, which are size-stable for up to 18 months. The first step of the synthesis involves a short sonication of gold (III) chloride hydrate with polyamidoamine dendrimers of the fourth generation, while the second step uses microwaves to reduce gold (III) chloride hydrate with sodium citrate. The developed synthesis method enables rapid production of spherical and monodisperse gold nanoparticles stabilized with polyamidoamine dendrimers. Physicochemical characterization of the gold nanoparticle-polyamidoamine dendrimers complexes was performed using ultraviolet-visible spectroscopy, dynamic light scattering technique, infrared spectroscopy, atomic force microscopy, and transmission electron microscopy. The toxicity of synthesized complexes on the breast cancer MCF-7 cell line has been studied using the tetrazolium salt reduction test. The produced gold nanoparticles revealed lower toxicity levels on the MCF-7 cell line after 18 months from synthesis compared with newly synthesized colloids. Synthesized gold nanoparticles stabilized with dendrimers and commercially available gold nanoparticles stabilized with sodium citrate show similar toxicity levels on breast cancer cells. |
| format | Article |
| id | doaj-art-b56390ccaa0f47618c0822afd2c7cddf |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-b56390ccaa0f47618c0822afd2c7cddf2025-08-20T02:59:08ZengMDPI AGMolecules1420-30492025-05-01309202410.3390/molecules30092024Evaluation of PAMAM Dendrimer-Stabilized Gold Nanoparticles: Two-Stage Procedure Synthesis and Toxicity Assessment in MCF-7 Breast Cancer CellsAgnieszka Maria Kołodziejczyk0Magdalena Grala1Łukasz Kołodziejczyk2Nanomaterial Structural Research Laboratory and Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Dubois 114/116, 93-465 Lodz, PolandNanomaterial Structural Research Laboratory and Molecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Dubois 114/116, 93-465 Lodz, PolandInstitute of Materials Science and Engineering, Lodz University of Technology, Stefanowskiego 1/15, 90-537 Lodz, PolandGold nanoparticles stabilized with polyamidoamine dendrimers are one of the potential candidates for use as a contrast agent in computed tomography and a drug delivery agent. This work demonstrates a rapid, two-step synthesis of such complexes, which are size-stable for up to 18 months. The first step of the synthesis involves a short sonication of gold (III) chloride hydrate with polyamidoamine dendrimers of the fourth generation, while the second step uses microwaves to reduce gold (III) chloride hydrate with sodium citrate. The developed synthesis method enables rapid production of spherical and monodisperse gold nanoparticles stabilized with polyamidoamine dendrimers. Physicochemical characterization of the gold nanoparticle-polyamidoamine dendrimers complexes was performed using ultraviolet-visible spectroscopy, dynamic light scattering technique, infrared spectroscopy, atomic force microscopy, and transmission electron microscopy. The toxicity of synthesized complexes on the breast cancer MCF-7 cell line has been studied using the tetrazolium salt reduction test. The produced gold nanoparticles revealed lower toxicity levels on the MCF-7 cell line after 18 months from synthesis compared with newly synthesized colloids. Synthesized gold nanoparticles stabilized with dendrimers and commercially available gold nanoparticles stabilized with sodium citrate show similar toxicity levels on breast cancer cells.https://www.mdpi.com/1420-3049/30/9/2024gold nanoparticles synthesisAuNPsPAMAM G4 dendrimersatomic force microscopyXTT tetrazolium salt reduction testscellular viability |
| spellingShingle | Agnieszka Maria Kołodziejczyk Magdalena Grala Łukasz Kołodziejczyk Evaluation of PAMAM Dendrimer-Stabilized Gold Nanoparticles: Two-Stage Procedure Synthesis and Toxicity Assessment in MCF-7 Breast Cancer Cells Molecules gold nanoparticles synthesis AuNPs PAMAM G4 dendrimers atomic force microscopy XTT tetrazolium salt reduction tests cellular viability |
| title | Evaluation of PAMAM Dendrimer-Stabilized Gold Nanoparticles: Two-Stage Procedure Synthesis and Toxicity Assessment in MCF-7 Breast Cancer Cells |
| title_full | Evaluation of PAMAM Dendrimer-Stabilized Gold Nanoparticles: Two-Stage Procedure Synthesis and Toxicity Assessment in MCF-7 Breast Cancer Cells |
| title_fullStr | Evaluation of PAMAM Dendrimer-Stabilized Gold Nanoparticles: Two-Stage Procedure Synthesis and Toxicity Assessment in MCF-7 Breast Cancer Cells |
| title_full_unstemmed | Evaluation of PAMAM Dendrimer-Stabilized Gold Nanoparticles: Two-Stage Procedure Synthesis and Toxicity Assessment in MCF-7 Breast Cancer Cells |
| title_short | Evaluation of PAMAM Dendrimer-Stabilized Gold Nanoparticles: Two-Stage Procedure Synthesis and Toxicity Assessment in MCF-7 Breast Cancer Cells |
| title_sort | evaluation of pamam dendrimer stabilized gold nanoparticles two stage procedure synthesis and toxicity assessment in mcf 7 breast cancer cells |
| topic | gold nanoparticles synthesis AuNPs PAMAM G4 dendrimers atomic force microscopy XTT tetrazolium salt reduction tests cellular viability |
| url | https://www.mdpi.com/1420-3049/30/9/2024 |
| work_keys_str_mv | AT agnieszkamariakołodziejczyk evaluationofpamamdendrimerstabilizedgoldnanoparticlestwostageproceduresynthesisandtoxicityassessmentinmcf7breastcancercells AT magdalenagrala evaluationofpamamdendrimerstabilizedgoldnanoparticlestwostageproceduresynthesisandtoxicityassessmentinmcf7breastcancercells AT łukaszkołodziejczyk evaluationofpamamdendrimerstabilizedgoldnanoparticlestwostageproceduresynthesisandtoxicityassessmentinmcf7breastcancercells |