Disrupted Redox Regulation and Inflammatory Response in Pyoderma Gangrenosum

Introduction. The pathophysiology of Pyoderma Gangrenosum (PG) involves altered innate and adaptive immunity, mutagenic and epigenetic changes, the autoinflammatory state, and the overexpression of cytokines. This study investigated the potential contribution of inflammation, redox signaling, and th...

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Main Authors: Simona Roxana Georgescu, Clara Matei, Corina Daniela Ene, Cristina Capusa, Mircea Tampa, Madalina Irina Mitran, Cristina Iulia Mitran, Gheorghe Nicolae, Ilinca Nicolae
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Life
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Online Access:https://www.mdpi.com/2075-1729/15/4/611
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author Simona Roxana Georgescu
Clara Matei
Corina Daniela Ene
Cristina Capusa
Mircea Tampa
Madalina Irina Mitran
Cristina Iulia Mitran
Gheorghe Nicolae
Ilinca Nicolae
author_facet Simona Roxana Georgescu
Clara Matei
Corina Daniela Ene
Cristina Capusa
Mircea Tampa
Madalina Irina Mitran
Cristina Iulia Mitran
Gheorghe Nicolae
Ilinca Nicolae
author_sort Simona Roxana Georgescu
collection DOAJ
description Introduction. The pathophysiology of Pyoderma Gangrenosum (PG) involves altered innate and adaptive immunity, mutagenic and epigenetic changes, the autoinflammatory state, and the overexpression of cytokines. This study investigated the potential contribution of inflammation, redox signaling, and the immune system in the pathogenesis of PG. Materials and Methods. This case–control study included 36 patients with PG and 30 controls. We have determined the serum concentrations of acute phase proteins (C-reactive protein—CRP, alpha1 glycoprotein acid—AGPA, Albumin), interleukin-17A -IL-17A, β2 microglobulin-β2MG, reduced glutathione-GSH, oxidized glutathione- GSSG, the GSH/GSSG ratio, and hematological parameters (white blood cells-WBC, neutrophil-lymphocyte ratio-NLR, erythrocyte sedimentation rate-ESR) in patients with PG compared with controls. Furthermore, we have evaluated the variations in these markers before and after treatment in PG patients. Results. The serum concentrations of acute phase proteins (CRP, AGPA, and Albumin) and the IL-17A, β2MG, GSH, GSSG, and GSH/GSSG ratio were significantly different between the PG group and controls. Hematological parameters (WBC, NLR, and ESR), acute phase proteins (CRP, AGPA, and albumin), and IL-17A showed an exaggerated and persistent inflammatory response in patients with PG. In patients with PG associated with systemic diseases, the dysregulation of the biochemical events was more severe. Conclusions. The acute phase proteins, β2MG-MHC class I complex, and the GSH-GSSG system are unbalanced in PG. Our results could improve the diagnosis and our understanding of the pathogenic basis of PG.
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spelling doaj-art-b49876bd55e44aa488ae7760f374a6e42025-08-20T02:28:15ZengMDPI AGLife2075-17292025-04-0115461110.3390/life15040611Disrupted Redox Regulation and Inflammatory Response in Pyoderma GangrenosumSimona Roxana Georgescu0Clara Matei1Corina Daniela Ene2Cristina Capusa3Mircea Tampa4Madalina Irina Mitran5Cristina Iulia Mitran6Gheorghe Nicolae7Ilinca Nicolae8Department of Dermatology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Dermatology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartments of Nephrology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartments of Nephrology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Dermatology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Microbiology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Microbiology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaFaculty of Psychology, Babeș-Bolyai University, 400347 Cluj-Napoca, RomaniaDepartment of Dermatology, ‘Victor Babes’ Clinical Hospital for Infectious Diseases, 030303 Bucharest, RomaniaIntroduction. The pathophysiology of Pyoderma Gangrenosum (PG) involves altered innate and adaptive immunity, mutagenic and epigenetic changes, the autoinflammatory state, and the overexpression of cytokines. This study investigated the potential contribution of inflammation, redox signaling, and the immune system in the pathogenesis of PG. Materials and Methods. This case–control study included 36 patients with PG and 30 controls. We have determined the serum concentrations of acute phase proteins (C-reactive protein—CRP, alpha1 glycoprotein acid—AGPA, Albumin), interleukin-17A -IL-17A, β2 microglobulin-β2MG, reduced glutathione-GSH, oxidized glutathione- GSSG, the GSH/GSSG ratio, and hematological parameters (white blood cells-WBC, neutrophil-lymphocyte ratio-NLR, erythrocyte sedimentation rate-ESR) in patients with PG compared with controls. Furthermore, we have evaluated the variations in these markers before and after treatment in PG patients. Results. The serum concentrations of acute phase proteins (CRP, AGPA, and Albumin) and the IL-17A, β2MG, GSH, GSSG, and GSH/GSSG ratio were significantly different between the PG group and controls. Hematological parameters (WBC, NLR, and ESR), acute phase proteins (CRP, AGPA, and albumin), and IL-17A showed an exaggerated and persistent inflammatory response in patients with PG. In patients with PG associated with systemic diseases, the dysregulation of the biochemical events was more severe. Conclusions. The acute phase proteins, β2MG-MHC class I complex, and the GSH-GSSG system are unbalanced in PG. Our results could improve the diagnosis and our understanding of the pathogenic basis of PG.https://www.mdpi.com/2075-1729/15/4/611pyoderma gangrenosumacute phase proteinsIL-17ABeta2 microglobulinglutathione
spellingShingle Simona Roxana Georgescu
Clara Matei
Corina Daniela Ene
Cristina Capusa
Mircea Tampa
Madalina Irina Mitran
Cristina Iulia Mitran
Gheorghe Nicolae
Ilinca Nicolae
Disrupted Redox Regulation and Inflammatory Response in Pyoderma Gangrenosum
Life
pyoderma gangrenosum
acute phase proteins
IL-17A
Beta2 microglobulin
glutathione
title Disrupted Redox Regulation and Inflammatory Response in Pyoderma Gangrenosum
title_full Disrupted Redox Regulation and Inflammatory Response in Pyoderma Gangrenosum
title_fullStr Disrupted Redox Regulation and Inflammatory Response in Pyoderma Gangrenosum
title_full_unstemmed Disrupted Redox Regulation and Inflammatory Response in Pyoderma Gangrenosum
title_short Disrupted Redox Regulation and Inflammatory Response in Pyoderma Gangrenosum
title_sort disrupted redox regulation and inflammatory response in pyoderma gangrenosum
topic pyoderma gangrenosum
acute phase proteins
IL-17A
Beta2 microglobulin
glutathione
url https://www.mdpi.com/2075-1729/15/4/611
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