Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine
The human intestine plays a pivotal role in nutrient absorption and immune system regulation. Along the longitudinal axis, cell-type composition changes to meet the varying functional requirements. Therefore, our protocol focuses on the processing of the whole human intestine to facilitate the analy...
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2025-01-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5157&type=0 |
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| author | Enrique Gamero-Estevez Inga Hensel Michelle Steinhauer Olivia Müllertz Elizaveta Savochkina Ibrahim Sektioglu Bilgenaz Stoll Shaghayegh Derakhshani Sarah Devriese Kyungbo Kim Martin Resnik-Docampo |
| author_facet | Enrique Gamero-Estevez Inga Hensel Michelle Steinhauer Olivia Müllertz Elizaveta Savochkina Ibrahim Sektioglu Bilgenaz Stoll Shaghayegh Derakhshani Sarah Devriese Kyungbo Kim Martin Resnik-Docampo |
| author_sort | Enrique Gamero-Estevez |
| collection | DOAJ |
| description | The human intestine plays a pivotal role in nutrient absorption and immune system regulation. Along the longitudinal axis, cell-type composition changes to meet the varying functional requirements. Therefore, our protocol focuses on the processing of the whole human intestine to facilitate the analysis of region-specific characteristics such as tissue architecture and changes in cell populations. We describe how to generate a biobank that can be used to isolate specific immune cell subtypes, generate organoid lines, and establish autologous immune cell-organoid co-cultures. |
| format | Article |
| id | doaj-art-b3f9aebe8070475cb502efd9d5e34189 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-b3f9aebe8070475cb502efd9d5e341892025-02-07T08:16:31ZengBio-protocol LLCBio-Protocol2331-83252025-01-0115110.21769/BioProtoc.5157Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human IntestineEnrique Gamero-Estevez0Inga Hensel1Michelle Steinhauer2Olivia Müllertz3Elizaveta Savochkina4Ibrahim Sektioglu5Bilgenaz Stoll6Shaghayegh Derakhshani7Sarah Devriese8Kyungbo Kim9Martin Resnik-Docampo10BioMed X Institute, Heidelberg, GermanyBioMed X Institute, Heidelberg, GermanyBioMed X Institute, Heidelberg, GermanyBioMed X Institute, Heidelberg, GermanyBioMed X Institute, Heidelberg, GermanyBioMed X Institute, Heidelberg, GermanyBioMed X Institute, Heidelberg, GermanyBioMed X Institute, Heidelberg, GermanyBioMed X Institute, Heidelberg, GermanyBioMed X Institute, Heidelberg, GermanyBioMed X Institute, Heidelberg, GermanyThe human intestine plays a pivotal role in nutrient absorption and immune system regulation. Along the longitudinal axis, cell-type composition changes to meet the varying functional requirements. Therefore, our protocol focuses on the processing of the whole human intestine to facilitate the analysis of region-specific characteristics such as tissue architecture and changes in cell populations. We describe how to generate a biobank that can be used to isolate specific immune cell subtypes, generate organoid lines, and establish autologous immune cell-organoid co-cultures.https://bio-protocol.org/en/bpdetail?id=5157&type=0 |
| spellingShingle | Enrique Gamero-Estevez Inga Hensel Michelle Steinhauer Olivia Müllertz Elizaveta Savochkina Ibrahim Sektioglu Bilgenaz Stoll Shaghayegh Derakhshani Sarah Devriese Kyungbo Kim Martin Resnik-Docampo Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine Bio-Protocol |
| title | Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine |
| title_full | Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine |
| title_fullStr | Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine |
| title_full_unstemmed | Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine |
| title_short | Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine |
| title_sort | protocol for immune cell isolation organoid generation and co culture establishment from cryopreserved whole human intestine |
| url | https://bio-protocol.org/en/bpdetail?id=5157&type=0 |
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