Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine

Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine

The human intestine plays a pivotal role in nutrient absorption and immune system regulation. Along the longitudinal axis, cell-type composition changes to meet the varying functional requirements. Therefore, our protocol focuses on the processing of the whole human intestine to facilitate the analy...

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Main Authors: Enrique Gamero-Estevez, Inga Hensel, Michelle Steinhauer, Olivia Müllertz, Elizaveta Savochkina, Ibrahim Sektioglu, Bilgenaz Stoll, Shaghayegh Derakhshani, Sarah Devriese, Kyungbo Kim, Martin Resnik-Docampo
Format: Article
Language:English
Published: Bio-protocol LLC 2025-01-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=5157&type=0
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