Assessing Bias and Reproducibility of Viral Metagenomics Methods for the Combined Detection of Faecal RNA and DNA Viruses

Assessing Bias and Reproducibility of Viral Metagenomics Methods for the Combined Detection of Faecal RNA and DNA Viruses

Whole transcriptome amplification (WTA2) and sequence-independent single primer amplification (SISPA) are two widely used methods for combined metagenomic sequencing of RNA and DNA viruses. However, information on the reproducibility and bias of these methods on diverse viruses in faecal samples is...

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Bibliographic Details
Main Authors: Rik Haagmans, Oliver J. Charity, Dave Baker, Andrea Telatin, George M. Savva, Evelien M. Adriaenssens, Penny P. Powell, Simon R. Carding
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Viruses
Subjects:
viral metagenomics
mock community
eukaryotic viruses
bacteriophages
Online Access:https://www.mdpi.com/1999-4915/17/2/155
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https://www.mdpi.com/1999-4915/17/2/155

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