Role of glucocorticoid receptor expression in Chronic Chagas Cardiomyopathy: implications for inflammation and cardiac hypertrophy
IntroductionChronic Chagasic Cardiomyopathy (CCC) has an infectious and inflammatory nature. Recent data also suggest an association with altered regulation of glucocorticoid (GC)-mediated circuits failing to control systemic inflammation. However, the involvement of glucocorticoid receptors (GR) an...
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Frontiers Media S.A.
2025-01-01
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| author | Florencia B. González Florencia B. González Antonella Pacini Araceli Castro Susana Lioi Silvina R. Villar Silvina R. Villar Luciano D’Attilio Luciano D’Attilio Rodolfo D. Leiva Rodolfo D. Leiva Liliana Favaloro Oscar A. Bottasso Oscar A. Bottasso Carlos A. Vigliano Carlos A. Vigliano Ana Rosa Pérez Ana Rosa Pérez |
| author_facet | Florencia B. González Florencia B. González Antonella Pacini Araceli Castro Susana Lioi Silvina R. Villar Silvina R. Villar Luciano D’Attilio Luciano D’Attilio Rodolfo D. Leiva Rodolfo D. Leiva Liliana Favaloro Oscar A. Bottasso Oscar A. Bottasso Carlos A. Vigliano Carlos A. Vigliano Ana Rosa Pérez Ana Rosa Pérez |
| author_sort | Florencia B. González |
| collection | DOAJ |
| description | IntroductionChronic Chagasic Cardiomyopathy (CCC) has an infectious and inflammatory nature. Recent data also suggest an association with altered regulation of glucocorticoid (GC)-mediated circuits failing to control systemic inflammation. However, the involvement of glucocorticoid receptors (GR) and their isoforms have been unexplored.Materials and methodsThe expression of GR-α/β isoforms, 11β-hydroxysteroid dehydrogenase type-1 (11β-HSD1), inflammatory cytokines, and the GC-regulated gene tristetraprolin (TTP) in peripheral blood mononuclear cells (PBMCs) as well as GR immunoreactivity in the myocardium from CCC individuals were evaluated by qPCR and immunohistochemistry respectively. Heart control samples with no evidence of structural heart disease and from ischemic cardiomyopathy patients were included. The presence of inflammatory infiltrates and fibrosis were also recorded.ResultsGR-α was expressed similarly in the PBMCs from Co and CCC individuals, but 11β-HSD1 expression was increased only in CCC, conjointly with enhanced ratios of IL-6/TTP and IFN-γ/TTP. In the inflamed myocardium from CCC patients, positive GR expression correlated with the intensity of the inflammatory infiltrate and cardiac hypertrophy.ConclusionThe infectious and inflammatory nature of CCC pathology seems strongly connected with the expression of GR in cardiac tissue samples, providing a stimulating background for further studies addressed to elucidate the influence of GR expression and function on CCC pathophysiology and cardiomyocyte hypertrophy. |
| format | Article |
| id | doaj-art-b35f5859cc584a5d8f0644b228bd18a7 |
| institution | Kabale University |
| issn | 1664-2392 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj-art-b35f5859cc584a5d8f0644b228bd18a72025-01-30T05:10:05ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922025-01-011610.3389/fendo.2025.14867721486772Role of glucocorticoid receptor expression in Chronic Chagas Cardiomyopathy: implications for inflammation and cardiac hypertrophyFlorencia B. González0Florencia B. González1Antonella Pacini2Araceli Castro3Susana Lioi4Silvina R. Villar5Silvina R. Villar6Luciano D’Attilio7Luciano D’Attilio8Rodolfo D. Leiva9Rodolfo D. Leiva10Liliana Favaloro11Oscar A. Bottasso12Oscar A. Bottasso13Carlos A. Vigliano14Carlos A. Vigliano15Ana Rosa Pérez16Ana Rosa Pérez17Instituto de Inmunología Clínica y Experimental de Rosario (IDICER-CONICET), Rosario, ArgentinaFacultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, ArgentinaInstituto de Inmunología Clínica y Experimental de Rosario (IDICER-CONICET), Rosario, ArgentinaInstituto de Medicina Traslacional, Trasplante y Bioingeniería (IMeTTyB), Universidad Favaloro-CONICET, Buenos Aires, ArgentinaLaboratorio Central del Hospital Provincial del Centenario, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, ArgentinaInstituto de Inmunología Clínica y Experimental de Rosario (IDICER-CONICET), Rosario, ArgentinaFacultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, ArgentinaInstituto de Inmunología Clínica y Experimental de Rosario (IDICER-CONICET), Rosario, ArgentinaFacultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, ArgentinaFacultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, ArgentinaServicio de Cardiología, Sección Chagas del Hospital Provincial del Centenario, Rosario, ArgentinaServicio de Insuficiencia Cardíaca y Trasplante Cardíaco, Hospital Universitario Fundación Favaloro, Buenos Aires, ArgentinaInstituto de Inmunología Clínica y Experimental de Rosario (IDICER-CONICET), Rosario, ArgentinaFacultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, ArgentinaInstituto de Medicina Traslacional, Trasplante y Bioingeniería (IMeTTyB), Universidad Favaloro-CONICET, Buenos Aires, ArgentinaServicio de Anatomía Patológica, Hospital Universitario de la Fundación Favaloro, Buenos Aires, ArgentinaInstituto de Inmunología Clínica y Experimental de Rosario (IDICER-CONICET), Rosario, ArgentinaFacultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, ArgentinaIntroductionChronic Chagasic Cardiomyopathy (CCC) has an infectious and inflammatory nature. Recent data also suggest an association with altered regulation of glucocorticoid (GC)-mediated circuits failing to control systemic inflammation. However, the involvement of glucocorticoid receptors (GR) and their isoforms have been unexplored.Materials and methodsThe expression of GR-α/β isoforms, 11β-hydroxysteroid dehydrogenase type-1 (11β-HSD1), inflammatory cytokines, and the GC-regulated gene tristetraprolin (TTP) in peripheral blood mononuclear cells (PBMCs) as well as GR immunoreactivity in the myocardium from CCC individuals were evaluated by qPCR and immunohistochemistry respectively. Heart control samples with no evidence of structural heart disease and from ischemic cardiomyopathy patients were included. The presence of inflammatory infiltrates and fibrosis were also recorded.ResultsGR-α was expressed similarly in the PBMCs from Co and CCC individuals, but 11β-HSD1 expression was increased only in CCC, conjointly with enhanced ratios of IL-6/TTP and IFN-γ/TTP. In the inflamed myocardium from CCC patients, positive GR expression correlated with the intensity of the inflammatory infiltrate and cardiac hypertrophy.ConclusionThe infectious and inflammatory nature of CCC pathology seems strongly connected with the expression of GR in cardiac tissue samples, providing a stimulating background for further studies addressed to elucidate the influence of GR expression and function on CCC pathophysiology and cardiomyocyte hypertrophy.https://www.frontiersin.org/articles/10.3389/fendo.2025.1486772/fullglucocorticoid receptor11β-hydroxysteroid dehydrogenase type 1cardiomyocytehypertrophychronic chagasic cardiomyopathyischemic cardiomyopathy |
| spellingShingle | Florencia B. González Florencia B. González Antonella Pacini Araceli Castro Susana Lioi Silvina R. Villar Silvina R. Villar Luciano D’Attilio Luciano D’Attilio Rodolfo D. Leiva Rodolfo D. Leiva Liliana Favaloro Oscar A. Bottasso Oscar A. Bottasso Carlos A. Vigliano Carlos A. Vigliano Ana Rosa Pérez Ana Rosa Pérez Role of glucocorticoid receptor expression in Chronic Chagas Cardiomyopathy: implications for inflammation and cardiac hypertrophy Frontiers in Endocrinology glucocorticoid receptor 11β-hydroxysteroid dehydrogenase type 1 cardiomyocyte hypertrophy chronic chagasic cardiomyopathy ischemic cardiomyopathy |
| title | Role of glucocorticoid receptor expression in Chronic Chagas Cardiomyopathy: implications for inflammation and cardiac hypertrophy |
| title_full | Role of glucocorticoid receptor expression in Chronic Chagas Cardiomyopathy: implications for inflammation and cardiac hypertrophy |
| title_fullStr | Role of glucocorticoid receptor expression in Chronic Chagas Cardiomyopathy: implications for inflammation and cardiac hypertrophy |
| title_full_unstemmed | Role of glucocorticoid receptor expression in Chronic Chagas Cardiomyopathy: implications for inflammation and cardiac hypertrophy |
| title_short | Role of glucocorticoid receptor expression in Chronic Chagas Cardiomyopathy: implications for inflammation and cardiac hypertrophy |
| title_sort | role of glucocorticoid receptor expression in chronic chagas cardiomyopathy implications for inflammation and cardiac hypertrophy |
| topic | glucocorticoid receptor 11β-hydroxysteroid dehydrogenase type 1 cardiomyocyte hypertrophy chronic chagasic cardiomyopathy ischemic cardiomyopathy |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2025.1486772/full |
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