Peptide fraction from sturgeon muscle by pepsin hydrolysis exerts anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via MAPK and NF-κB pathways
Previous studies have suggested that polypeptides extracted from milk, soybean, fish, eggs, and meat possess potential anti-inflammatory effects. To date, few studies have reported the anti-inflammatory function of sturgeon peptides and their underlying mechanisms are unknown. The current study was...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2021-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453020301452 |
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| author | Ruichang Gao Wanghui Shu Yang Shen Quancai Sun Wengang Jin Dajing Li Ying Li Li Yuan |
| author_facet | Ruichang Gao Wanghui Shu Yang Shen Quancai Sun Wengang Jin Dajing Li Ying Li Li Yuan |
| author_sort | Ruichang Gao |
| collection | DOAJ |
| description | Previous studies have suggested that polypeptides extracted from milk, soybean, fish, eggs, and meat possess potential anti-inflammatory effects. To date, few studies have reported the anti-inflammatory function of sturgeon peptides and their underlying mechanisms are unknown. The current study was therefore to determine the anti-inflammatory potential of sturgeon peptides with lipopolysaccharide (LPS)-induced RAW264.7 inflammatory model. Pepsin hydrolysate (PeH) was purified by ultrafiltration and Sephadex G-15 gel filtration chromatography. PeH significantly reduced the inflammatory mediator (NO) and inflammatory cytokines (IL-6, TNF-α and IL-1β) expression in a dose-dependent manner. Moreover, the purified sturgeon peptide (F2) possessed strong antioxidant potential and effectively inhibited DPPH and ABTS free radicals. F2 significantly suppressed the expression of MAPK, IκBα, and NF-κB p65, indicating that F2 exerted anti-inflammatory influence by the inhibition of MAPK and NF-κB pathways. |
| format | Article |
| id | doaj-art-b34573df6291402982f5fd47b79293ee |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-b34573df6291402982f5fd47b79293ee2025-02-03T02:33:32ZengTsinghua University PressFood Science and Human Wellness2213-45302021-01-01101103111Peptide fraction from sturgeon muscle by pepsin hydrolysis exerts anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via MAPK and NF-κB pathwaysRuichang Gao0Wanghui Shu1Yang Shen2Quancai Sun3Wengang Jin4Dajing Li5Ying Li6Li Yuan7School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province, 212013, China; Bio-resources Key Laboratory of Shaanxi Province, School of Bioscience and Engineering, Shaanxi University of Technology, Hanzhong, 723001, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province, 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province, 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province, 212013, ChinaBio-resources Key Laboratory of Shaanxi Province, School of Bioscience and Engineering, Shaanxi University of Technology, Hanzhong, 723001, ChinaInstitute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, ChinaInstitute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province, 212013, China; Corresponding author at: School of Food and Biological Engineering, Jiangsu University, No. 301, Xuefu Road, Zhenjiang, Jiangsu Province, 212013, China.Previous studies have suggested that polypeptides extracted from milk, soybean, fish, eggs, and meat possess potential anti-inflammatory effects. To date, few studies have reported the anti-inflammatory function of sturgeon peptides and their underlying mechanisms are unknown. The current study was therefore to determine the anti-inflammatory potential of sturgeon peptides with lipopolysaccharide (LPS)-induced RAW264.7 inflammatory model. Pepsin hydrolysate (PeH) was purified by ultrafiltration and Sephadex G-15 gel filtration chromatography. PeH significantly reduced the inflammatory mediator (NO) and inflammatory cytokines (IL-6, TNF-α and IL-1β) expression in a dose-dependent manner. Moreover, the purified sturgeon peptide (F2) possessed strong antioxidant potential and effectively inhibited DPPH and ABTS free radicals. F2 significantly suppressed the expression of MAPK, IκBα, and NF-κB p65, indicating that F2 exerted anti-inflammatory influence by the inhibition of MAPK and NF-κB pathways.http://www.sciencedirect.com/science/article/pii/S2213453020301452SturgeonEnzymatic hydrolysisAntioxidantAnti-inflammation mechanismRAW264.7 macrophages |
| spellingShingle | Ruichang Gao Wanghui Shu Yang Shen Quancai Sun Wengang Jin Dajing Li Ying Li Li Yuan Peptide fraction from sturgeon muscle by pepsin hydrolysis exerts anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via MAPK and NF-κB pathways Food Science and Human Wellness Sturgeon Enzymatic hydrolysis Antioxidant Anti-inflammation mechanism RAW264.7 macrophages |
| title | Peptide fraction from sturgeon muscle by pepsin hydrolysis exerts anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via MAPK and NF-κB pathways |
| title_full | Peptide fraction from sturgeon muscle by pepsin hydrolysis exerts anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via MAPK and NF-κB pathways |
| title_fullStr | Peptide fraction from sturgeon muscle by pepsin hydrolysis exerts anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via MAPK and NF-κB pathways |
| title_full_unstemmed | Peptide fraction from sturgeon muscle by pepsin hydrolysis exerts anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via MAPK and NF-κB pathways |
| title_short | Peptide fraction from sturgeon muscle by pepsin hydrolysis exerts anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via MAPK and NF-κB pathways |
| title_sort | peptide fraction from sturgeon muscle by pepsin hydrolysis exerts anti inflammatory effects in lps stimulated raw264 7 macrophages via mapk and nf κb pathways |
| topic | Sturgeon Enzymatic hydrolysis Antioxidant Anti-inflammation mechanism RAW264.7 macrophages |
| url | http://www.sciencedirect.com/science/article/pii/S2213453020301452 |
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