Construction of DF-1 Cell Line of Stably Expressing Cas9 Protein Mediated by Lentivirus and Verification of Its Activity
【Objective】The chicken fibroblast cell line (DF-1) of stably expressing Cas9 protein was screened, and combined with the reporter vector system based on single strand annealing (SSA) repair mechanism, the Cas9 nuclease activity in the established DF-1 cell line was detected.【Method】The lentiviral ve...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Guangdong Academy of Agricultural Sciences
2023-02-01
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| Series: | Guangdong nongye kexue |
| Subjects: | |
| Online Access: | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202302014 |
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