Cas9-based enrichment for targeted long-read metabarcoding

Metabarcoding is a valuable tool for characterizing the communities that underpin the functioning of ecosystems. However, current methods often rely on polymerase chain reaction (PCR) amplification for enrichment of marker genes. PCR can introduce significant biases that affect quantification and is...

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Bibliographic Details
Main Authors: Lucia Nikolaeva-Reynolds, Christopher Cammies, Rosemary Crichton, Thomas E. Gorochowski
Format: Article
Language:English
Published: The Royal Society 2025-04-01
Series:Royal Society Open Science
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Online Access:https://royalsocietypublishing.org/doi/10.1098/rsos.242110
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